1615-9314/asset/2259_left.gif?v=1&s=c1c1a836adaa97a8f341244e394b257c5efe2e29)
Short Communication
GC separation of amino acid enantiomers via derivatization with heptafluorobutyl chloroformate and Chirasil-L-Val column
Article first published online: 19 OCT 2009
DOI: 10.1002/jssc.200900400
Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Zahradníčková, H., Hušek, P. and Šimek, P. (2009), GC separation of amino acid enantiomers via derivatization with heptafluorobutyl chloroformate and Chirasil-L-Val column. Journal of Separation Science, 32: 3919–3924. doi: 10.1002/jssc.200900400
Publication History
- Issue published online: 23 DEC 2009
- Article first published online: 19 OCT 2009
- Manuscript Revised: 17 AUG 2009
- Manuscript Accepted: 17 AUG 2009
- Manuscript Received: 3 JUN 2009
Funded by
- Agency of the Czech Republic. Grant Number: 203/09/2014
- Abstract
- Article
- References
- Cited By
Keywords:
- Chiral separation;
- Derivatization;
- D,L-AAs;
- GC;
- HFBCF
Abstract
Heptafluorobutyl chloroformate (HFBCF), a recently introduced derivatization reagent, was examined in enantioseparation of amino acids (AAs) by GC. Twenty proteinogenic AAs, plus ornithine, cystine and 4-fluorophenylalanine (internal standard) were treated with the reagent and separation properties of the derivatives were assessed on a Chirasil-Val capillary column. Nineteen AA enantiomers were efficiently separated in 43 min except proline, arginine and cystine. The HFBCF derivatives of the studied DL-AAs show improved separation over other chloroformate-based derivatives hitherto reported. A combination of the improved and faster separation with a simple derivatization protocol, involving an immediate one-step reaction–extraction in two-phase aqueous-organic medium, and low elution temperatures extend application of HFBCF to chiral AA analysis.