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Improving the knowledge on Piper betle: Targeted metabolite analysis and effect on acetylcholinesterase

Authors

  • Patrícia Valentão,

    Corresponding author
    1. REQUIMTE/Department of Pharmacognosy, Faculty of Pharmacy, Porto University, Porto, Portugal
    • REQUIMTE/Department of Pharmacognosy, Faculty of Pharmacy, Porto University, R. Aníbal Cunha 164, 4050-047 Porto, Portugal Fax: +351-222003977
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  • Rui F. Gonçalves,

    1. REQUIMTE/Department of Pharmacognosy, Faculty of Pharmacy, Porto University, Porto, Portugal
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  • Cristóvão Belo,

    1. REQUIMTE/Department of Pharmacognosy, Faculty of Pharmacy, Porto University, Porto, Portugal
    2. Faculdade de Ciências da Educação, Universidade Nacional Timor Lorosa'e, Dili, Timor Lorosa'e
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  • Paula Guedes de Pinho,

    1. REQUIMTE/Department of Toxicology, Faculty of Pharmacy, Porto University, Porto, Portugal
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  • Paula B. Andrade,

    1. REQUIMTE/Department of Pharmacognosy, Faculty of Pharmacy, Porto University, Porto, Portugal
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  • Federico Ferreres

    1. Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), Campus University Espinardo, Murcia, Spain
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Abstract

Piper betle is a species growing in South East Asia, where its leaves are economically and medicinally important. To screen the highest possible number of volatile and semivolatile components, the leaves were subjected to headspace solid-phase microextraction, hydrodistillation and Soxhlet extraction, prior to analysis by GC/MS. Fifty compounds (identified by comparison with standard compounds or tentatively by National Institute of Standards and Technology database) were determined, 23 being described for the first time in this matrix. An aqueous extract was also analysed, in which only seven compounds were characterized. The organic acids' composition of this extract was determined by HPLC/UV and eight compounds are reported for the first time in P. betle. This extract also displayed acetylcholinesterase inhibitory capacity.

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