Liquid Chromatography

Development of a rapid and convenient method to separate eight ginsenosides from Panax ginseng by high-speed counter-current chromatography coupled with evaporative light scattering detection

  1. Omer Shehzad1,
  2. In Jin Ha1,
  3. Youmie Park2,
  4. Young Wan Ha3,
  5. Yeong Shik Kim1,*

Article first published online: 13 APR 2011

DOI: 10.1002/jssc.201000932

Issue

Journal of Separation Science

Journal of Separation Science

Volume 34, Issue 10, pages 1116–1122, May 2011

Additional Information

How to Cite

Shehzad, O., Jin Ha, I., Park, Y., Wan Ha, Y. and Shik Kim, Y. (2011), Development of a rapid and convenient method to separate eight ginsenosides from Panax ginseng by high-speed counter-current chromatography coupled with evaporative light scattering detection. Journal of Separation Science, 34: 1116–1122. doi: 10.1002/jssc.201000932

Author Information

  1. 1

    College of Pharmacy, Natural Products Research Institute, Seoul National University, Seoul, Korea

  2. 2

    College of Pharmacy, Inje University, Gimhae, Gyeongnam, Korea

  3. 3

    Life/Health Division, Korea Institute of Science and Technology, Seoul, Korea

*College of Pharmacy, Natural Products Research Institute, Seoul National University, 599 Gwanagno, Gwanak-gu, Seoul 151-742, Korea Fax: +82-2-765-4768

Publication History

  1. Issue published online: 5 MAY 2011
  2. Article first published online: 13 APR 2011
  3. Manuscript Accepted: 25 FEB 2011
  4. Manuscript Revised: 15 FEB 2011
  5. Manuscript Received: 30 DEC 2010

Funded by

  • National Research Foundation of Korea
  • Basic Research Promotion Fund. Grant Number: 2010-0008384
  • Korean government (MEST). Grant Number: 20090083533

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Keywords:

  • Ginsenosides;
  • High-speed counter-current chromatography;
  • Panax ginseng;
  • Protopanaxadiol;
  • Protopanaxatriol

Abstract

Ginsenosides exhibit diverse biological activities and are major well-known components isolated from the radix of Panax ginseng C.A. Meyer. In the present work, a rapid and facile method for the separation and purification of eight ginsenosides from P. ginseng by high-speed counter-current chromatography coupled with evaporative light scattering detector (HSCCC-ELSD) was successfully developed. The crude samples for HSCCC separation were first purified from ginseng extract using a macroporous resin; the extract was loaded onto a Diaion-HP20 column and fractionated by methanol and water gradient elution. The ginsenosides-protopanaxadiol (PPD) and protopanaxatriol (PPT) fractions were subsequently eluted with 65 and 80% methanol and water gradient elution, respectively. Furthermore, these two fractions were separated by HSCCC-ELSD. The two-phase solvent system used for separation was composed of chloroform/methanol/water/isopropanol at a volume ratio of 4:3:2:1. Each fraction obtained was collected and dried, yielding the following eight ginsenosides: Rg1, Re, Rf, Rh1, Rb1, Rc Rb2 and Rd. The purity of these ginsenosides was greater than 97% as assessed by HPLC-ELSD, and their structures were characterized by electrospray-ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance spectroscopy. This is the first report regarding the separation of the ginsenosides Rh1, Rb2 and Rc from P. ginseng by HSCCC.

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