Silica-based and organic monolithic capillary columns for LC: Recent trends in proteomics

Authors

  • Johan Rozenbrand,

    Corresponding author
    1. Biomolecular Analysis, Faculty of Science, Department of Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands
    • Biomolecular Analysis, Faculty of Science, Department of Pharmaceutical Sciences, Utrecht University, Universiteitsweg 99, 3584 GG Utrecht, The Netherlands Fax: +31302536655
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  • Wouter P. van Bennekom

    1. Biomolecular Analysis, Faculty of Science, Department of Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands
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Abstract

The use of monolithic liquid chromatography (LC) columns for proteomics, covering the scientific literature from 2004 to the beginning of 2011, is reviewed. Attention is paid to recent developments in column technology and materials, focusing on silica-based and organic (polystyrene and methacrylate) monolithic capillary columns for proteomics. The applicability of these columns is illustrated by examples of the analysis of (complex) protein digests and proteins conveniently summarized in tables. Furthermore, characteristics of column materials are compared and future trends and prospects are presented.

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