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Feasibility of ultra high performance supercritical neat carbon dioxide chromatography at conventional pressures
Article first published online: 24 AUG 2011
DOI: 10.1002/jssc.201100332
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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How to Cite
Sarazin, C., Thiébaut, D., Sassiat, P. and Vial, J. (2011), Feasibility of ultra high performance supercritical neat carbon dioxide chromatography at conventional pressures. J. Sep. Science, 34: 2773–2778. doi: 10.1002/jssc.201100332
Publication History
- Issue published online: 22 SEP 2011
- Article first published online: 24 AUG 2011
- Manuscript Accepted: 9 JUL 2011
- Manuscript Revised: 8 JUL 2011
- Manuscript Received: 12 APR 2011
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Keywords:
- Sub-2 μm particles;
- Supercritical fluid chromatography;
- Ultra high performance liquid chromatography
Abstract
The implementation of columns packed with sub-2 μm particles in supercritical fluid chromatography (SFC) is described using neat carbon dioxide as the mobile phase. A conventional supercritical fluid chromatograph was slightly modified to reduce extra column band broadening. Performances of a column packed with 1.8 μm C18-bonded silica particles in SFC using neat carbon dioxide as the mobile phase were compared with results obtained in ultra high performance liquid chromatography (UHPLC) using a dedicated chromatograph. As expected and usual in SFC, higher linear velocities than in UHPLC must be applied in order to reach optimal efficiency owing to higher diffusion coefficient of solutes in the mobile phase; similar numbers of theoretical plates were obtained with both techniques. Very fast separations of hydrocarbons are presented using two different alkyl-bonded silica columns.