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Fingerprinting and simultaneous determination of alkaloids in Picrasma quassioides from different locations by high performance liquid chromatography with photodiode array detection

Authors

  • Hui-Jun Liao,

    1. New Drug Research & Development Center, Guangzhou University of Chinese Medicine, Guangzhou, P. R. China
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    • These authors have contributed equally to this work.

  • Zheng-Quan Lai,

    1. New Drug Research & Development Center, Guangzhou University of Chinese Medicine, Guangzhou, P. R. China
    2. School of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong SAR, P. R. China
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    • These authors have contributed equally to this work.

  • Ji-Yan Su,

    1. New Drug Research & Development Center, Guangzhou University of Chinese Medicine, Guangzhou, P. R. China
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  • Yu-Yang Yi,

    1. New Drug Research & Development Center, Guangzhou University of Chinese Medicine, Guangzhou, P. R. China
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  • Yu-Cui Li,

    1. New Drug Research & Development Center, Guangzhou University of Chinese Medicine, Guangzhou, P. R. China
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  • Xiao-Ping Lai,

    1. New Drug Research & Development Center, Guangzhou University of Chinese Medicine, Guangzhou, P. R. China
    2. Dongguan Mathematical Engineering and Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Dongguan, P. R. China
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  • Zi-Ren Su,

    Corresponding author
    • New Drug Research & Development Center, Guangzhou University of Chinese Medicine, Guangzhou, P. R. China
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  • Zhi-Xiu Lin

    Corresponding author
    • School of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong SAR, P. R. China
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Correspondence: Professor Zi-Ren Su, New Drug Research & Development Center, Guangzhou University of Chinese Medicine, 232 Wai Huan East Road, Guangzhou Higher Education Mega Center, Guangzhou 510006, P. R. China

E-mail: suziren@gzhtcm.edu.cn

Fax: +86 20 3935 8390

Additional correspondence: Professor Zhi-Xiu Lin, School of Chinese Medicine, 1/F., Sino Building, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, China, E-mail: linzx@cuhk.edu.hk

Abstract

A simple and sensitive method was developed and validated for profiling and simultaneous quantitation of seven alkaloids (6-hydroxy-β-carboline-1-carboxylic acid, β-carboline-1-carboxylic acid, β-carboline-1-propanoic acid, 3-methylcanthin-5,6-dione, 5-hydroxy-4-methoxycanthin-6-one, 1-methoxycarbony-β-carboline, and 4,5-dimethoxycanthin-6-one) in Picrasma quassioide grown in different locations by high-performance liquid chromatography with photodiode array detection. The analysis was conducted on a Phenomenex Gemini C18 column at 35°C with mobile phase of 25 mM aqueous ammonium acetate (pH 4.0, adjusted by glacial acetate acid) and acetonitrile. A common fingerprint chromatograph under 254 nm consisting of 27 peaks was constructed for the evaluation of the similarities among 31 P. quassioide samples. Samples from Guangdong and Guangxi Provinces were found to be within group linkage and showed significant difference from that of Jiangxi Province origin by using principal component analysis and hierarchical clustering analysis. In addition, the seven alkaloids were identified by electrospray ionization mass spectrometry and comparing with reference standards and literature data. All of them were determined simultaneously using the established HPLC method. This rapid and effective analytical method could be employed for quality assessment of P. quassioide, as well as pharmaceutical products containing this herbal material.

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