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Optimization of ultrasonic-assisted extraction of 3-monochloropropane-1,2-diol (MCPD) and analysis of its esters from edible oils by gas chromatography–mass spectrometry

Authors

  • Fei Ma,

    1. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, P. R. China
    2. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, P. R. China
    3. Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, P. R. China
    4. Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan, P. R. China
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    • Both Fei Ma and Peiwu Li are the first authors.

  • Peiwu Li,

    Corresponding author
    1. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, P. R. China
    2. Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, P. R. China
    3. Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, P. R. China
    • Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, P. R. China
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    • Both Fei Ma and Peiwu Li are the first authors.

  • Bertrand Matthäus,

    1. Max Rubner-Institut, Federal Research Institute for Nutrition and Food, Detmold, Germany
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  • Wen Zhang,

    1. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, P. R. China
    2. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, P. R. China
    3. Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, P. R. China
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  • Qi Zhang

    1. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, P. R. China
    2. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, P. R. China
    3. Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, P. R. China
    4. Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan, P. R. China
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  • Colour Online: See the article online to view Figs. 2 and 8 in colour.

Correspondence: Dr. Peiwu Li, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China

E-mail: peiwuli@oilcrops.cn

Fax: +86-27-86812862

Abstract

In this paper, ultrasonic-assisted extraction of 3-chloropropane-1,2-diol and its esters from edible oils was studied with isotope dilution GC-MS. Effects of several experimental parameters, such as types and concentrations of extracting solvent, ratios of liquid to material, extraction temperature, time of ultrasonic treatment on the extraction efficiency of 3-chloropropane-1,2-diol and its esters from edible oils and sample preparation for calibration were compared and optimized. The optimal extraction conditions were suggested as 66 mg oil sample in mixture of 0.5 mL MTBE/ethyl acetate (20% v/v) and 0.5 mL of sulfuric acid/n-propanol (0.3% v/v), being extracted for 30 min at 45°C under ultrasonic irradiation. Good linearity was gained in the range of 0.020–5.000 μg/g with the limit of detection (LOD) of 0.006 μg/g (S/N = 3) and the limit of quantification (LOQ) of 0.020 μg/g (S/N = 10). The recoveries at five spiked concentrations were ranged from 91.9 to 109.3% with RSD less than 9.4%. The method was successfully applied to the determination of 3-chloropropane-1,2-diol and its esters amounts in rapeseed, sesame, peanut, camellia, and soybean oils.

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