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Fast separations of intact proteins by liquid chromatography

Authors

  • Fei Wang,

    1. Provincial Key Laboratory of Modern Separation Science of Shaanxi, Institute of Modern Separation Science, Northwest University, Xi'an, P. R. China
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  • Yi Min,

    1. Provincial Key Laboratory of Modern Separation Science of Shaanxi, Institute of Modern Separation Science, Northwest University, Xi'an, P. R. China
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  • Xindu Geng

    Corresponding author
    1. Provincial Key Laboratory of Modern Separation Science of Shaanxi, Institute of Modern Separation Science, Northwest University, Xi'an, P. R. China
    • Correspondence: Prof. Xindu Geng, Provincial Key Laboratory of Modern Separation Science of Shaanxi, Institute of Modern Separation Science, Northwest University, Xi'an 710069, P. R. China

      E-mail: xdgeng@nwu.edu.cn

      Fax: + 86-029-88303817

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Abstract

Scientists working in many fields require fast separations of intact proteins using liquid chromatography. The fast separations here concern not only the separation step alone but also the complete chromatographic process, including column regeneration, system equilibration, and buffer exchange, in one- and two-dimensional liquid chromatography in addition to fast purification technologies predominantly on the analytical scale with some unique examples on the preparative and industrial scales. This comprehensive review discusses recent developments in methodologies, packing materials, column techniques, and purification technologies in the field of rapid liquid chromatography of intact proteins. Some typical examples are summarized in the tables.

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