Stacking and simultaneous determination of estrogens in water samples by CE with electrochemical detection

Authors

  • Ping Li,

    1. Department of Environmental Science and Shanghai Key Laboratory for Urban Ecology and Sustainability, East China Normal University, Shanghai, P. R. China
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  • Wenjie Zhao,

    1. Department of Environmental Science and Shanghai Key Laboratory for Urban Ecology and Sustainability, East China Normal University, Shanghai, P. R. China
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  • Xiaoning Wang,

    1. Department of Environmental Science and Shanghai Key Laboratory for Urban Ecology and Sustainability, East China Normal University, Shanghai, P. R. China
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  • Guoyue Shi,

    1. Department of Chemistry, East China Normal University, Shanghai, P. R. China
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  • Tianshu Zhou

    Corresponding author
    • Department of Environmental Science and Shanghai Key Laboratory for Urban Ecology and Sustainability, East China Normal University, Shanghai, P. R. China
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Correspondence: Professor Tianshu Zhou, Department of Environmental Science and Shanghai Key Laboratory for Urban Ecology and Sustainability, East China Normal University, 500 Dongchuan Road, Shanghai 200241, P. R. China

E-mail: tszhou@des.ecnu.edu.cn

Fax: 86-21-54341277

Abstract

A rapid and sensitive method based on transient ITP and field enhancement in CE with electrochemical detection at copper disk electrode was developed for the simultaneous separation and determination of three estrogens: estrone, 17β-estradiol, and estriol. The effects of several important factors that influence the separation and detection were investigated. Under the optimum conditions, the estrogens could be separated in 0.06 mol/L sodium hydroxide solution within 14 min. With transient ITP by addition of 0.5% NaCl, a good linear response was obtained for three estrogens from 0.2 to 10 μmol/L, with correlation coefficients higher than 0.9993. The detection limits were 8.9 × 10−8, 6.7 × 10−8, and 1.1 × 10−7 mol/L (S/N = 3) for estriol, 17β-estradiol, and estrone, respectively. This method was successfully employed to analyze different water samples from waterworks, tap water, fishpond, and river samples with recoveries in the range of 90.8–108.9%, and RSDs < 4.69%. The satisfied results demonstrated that this method was of convenient preparation, high sensitivity, and good repeatability, which could be applied to the rapid determination of environmental water samples.

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