Preparation and application of novel zwitterionic monolithic column for hydrophilic interaction chromatography

Authors

  • Zhenghua Liu,

    1. Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, China
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    • These authors contributed equally to this work

  • Yongbo Peng,

    1. Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, China
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    • These authors contributed equally to this work

  • Tingting Wang,

    1. Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, China
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  • Guangxin Yuan,

    1. Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, China
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  • Qiaoxuan Zhang,

    1. Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, China
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  • Jialiang Guo,

    1. Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, China
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  • Zhengjin Jiang

    Corresponding author
    • Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, China
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  • This paper is included in the virtual special issue Monoliths available at the Journal of Separation Science website.

Correspondence: Dr. Zhengjin Jiang, School of Pharmacy, Jinan University, Huangpu Avenue West 601, Guangzhou 510632, China

E-mail: jzjjackson@hotmail.com

Fax: +86 2085224766

Abstract

A novel zwitterionic hydrophilic porous monolithic stationary phase was prepared based on the thermal-initiated copolymerization of N,N-dimethyl-N-(3-methacryl-amidopropyl)-N-(3-(sulfopropyl)ammonium betaine and ethylene glycol dimethacrylate. A typical hydrophilic separation mechanism was observed at a highly organic mobile phase (ACN >60%) on this optimized zwitterionic hydrophilic interaction chromatography (HILIC) monolithic stationary phase. Good permeability, stability, and column efficiency were observed on the final monolithic column. Additionally, a weak electrostatic interaction for charged analytes was confirmed in analysis of six benzoic acids by studying the influence of mobile phase pH and salt concentration on their retention behaviors on the obtained zwitterionic HILIC monolithic column. The optimized zwitterionic HILIC monolith exhibited good selectivity for a range of polar test analytes.

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