Enantiomeric separations by means of nano-LC

Authors

  • Anna Rocco,

    1. Department of Biochemistry and Biotechnologies, Vytautas Magnus University, Kaunas, Lithuania
    2. Institute of Chemical Methodologies, Consiglio Nazionale delle Ricerche, Rome, Italy
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  • Audrius Maruška,

    Corresponding author
    • Department of Biochemistry and Biotechnologies, Vytautas Magnus University, Kaunas, Lithuania
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  • Salvatore Fanali

    Corresponding author
    1. Institute of Chemical Methodologies, Consiglio Nazionale delle Ricerche, Rome, Italy
    • Department of Biochemistry and Biotechnologies, Vytautas Magnus University, Kaunas, Lithuania
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Correspondence: Dr. Salvatore Fanali, Institute of Chemical Methodologies, Consiglio Nazionale delle Ricerche, Via Salaria Km 29.300, 00015 Monterotondo, Rome, Italy

E-mail: salvatore.fanali@cnr.it

Fax: +390690672269

Additional Correspondence: Professor Audrius Maruška, E-mail: a.maruska@gmf.vdu.lt

Abstract

Enantiomers represent a class of compounds extensively investigated since they can show totally different behaviors when they interact with a chiral environment. Because of their identical chemical structure (they differ only in the spatial arrangement of the atoms in the molecule), the separation of optical isomers is a challenging task of analytical chemistry. So far employed methods for the separation of enantiomers are mainly based on chromatography. CE as well was considered as an analytical technique suitable for chiral separations, characterized by high efficiency and low consumption of reagent. Recently, miniaturization was introduced in LC to answer the needs to perform analyses in the minimum time, to use the smallest amount of samples and to reduce environmental pollution. Nano-LC represents nowadays a valid alternative to the abovementioned conventional analytical techniques, and can be advantageously exploited for enantiomeric separation especially because it needs minute amounts of the chiral material necessary to carry out enantiomeric separations. This review describes the development and applications of nano-LC in the field of chiral separations. The data reported in literature show its relevance for the study enantiomers-chiral selectors interaction, as well as for application in pharmaceutical and clinical research.

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