Research Article

Surface initiated polymerization of a cationic monomer on inner surfaces of silica capillaries: Analyte separation by capillary electrophoresis versus polyelectrolyte behavior

  1. Joanna Witos1,
  2. Mikko Karesoja2,
  3. Erno Karjalainen2,
  4. Heikki Tenhu2,†,
  5. Marja-Liisa Riekkola1,*

Article first published online: 18 FEB 2013

DOI: 10.1002/jssc.201200945

Issue

Journal of Separation Science

Journal of Separation Science

Volume 36, Issue 6, pages 1070–1077, March 2013

Additional Information

How to Cite

Witos, J., Karesoja, M., Karjalainen, E., Tenhu, H. and Riekkola, M.-L. (2013), Surface initiated polymerization of a cationic monomer on inner surfaces of silica capillaries: Analyte separation by capillary electrophoresis versus polyelectrolyte behavior. J. Sep. Science, 36: 1070–1077. doi: 10.1002/jssc.201200945

Author Information

  1. 1

    Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Helsinki, Finland

  2. 2

    Laboratory of Polymer Chemistry, Department of Chemistry, University of Helsinki, Helsinki, Finland

  1. †

    Additional correspondence: Professor Heikki Tenhu, E-mail: heikki.tenhu@helsinki.fi

* Correspondence: Professor Marja-Liisa Riekkola, Laboratory of Analytical Chemistry, Department of Chemistry, P.O. Box 55, FIN-00014, University of Helsinki, Helsinki, Finland
E-mail: marja-liisa.riekkola@helsinki.fi
Fax: +358-9-191-50253

  1. †

    Additional correspondence: Professor Heikki Tenhu, E-mail: heikki.tenhu@helsinki.fi

Publication History

  1. Issue published online: 13 MAR 2013
  2. Article first published online: 18 FEB 2013
  3. Accepted manuscript online: 7 JAN 2013 05:56AM EST
  4. Manuscript Accepted: 19 DEC 2012
  5. Manuscript Revised: 28 NOV 2012
  6. Manuscript Received: 10 OCT 2012

Funded by

  • National Doctoral Programme in Nanoscience
  • Research Council for Natural Sciences and Engineering of the Academy of Finland. Grant Number: 1133184
  • Magnus Ehrnrooth Foundation
  • National Center of Excellence of Functional Materials

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Keywords:

  • β-Blockers;
  • Basic proteins;
  • Capillary electrophoresis;
  • Lipoproteins;
  • Poly([2-(methacryloyl)oxyethyl]trimethylammonium chloride

[2-(Methacryloyl)oxyethyl]trimethylammonium chloride was successfully polymerized by surface-initiated atom transfer radical polymerization method on the inner surface of fused-silica capillaries resulting in a covalently bound poly([2-(methacryloyl)oxyethyl]trimethylammonium chloride) coating. The coated capillaries provided in capillary electrophoresis an excellent run-to-run repeatability, capillary-to-capillary and day-to-day reproducibility. The capillaries worked reliably over 1 month with EOF repeatability below 0.5%. The positively charged coated capillaries were successfully applied to the capillary electrophoretic separation of three standard proteins and five β-blockers with the separation efficiencies ranging from 132 000 to 303 000 plates/m, and from 82 000 to 189 000 plates/m, respectively. In addition, challenging high- and low-density lipoprotein particles could be separated. The hydrodynamic sizes of free polymer chains in buffers used in the capillary electrophoretic experiments were measured for the characterization of the coatings.

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