Separation of peptides with an aqueous mobile phase by temperature-responsive chromatographic column (J. Sep. Science 35'16)
Rongji Dai, Zongjian Liu, Yanli Liang, Fangfang Geng, Chen Ge, Kaleem Ullah, Fang Lv, Yukui Zhang and Yulin Deng
Article first published online: 16 AUG 2012 | DOI: 10.1002/jssc.201270122
A thermoresponsive chromatographic material was prepared through a simple method and employed for the temperature-modulated separation of peptides in an aqueous mobile phase. At 10°C, the grafted polymer chains are hydrophilic and in the form of extended coil. In this case, the peptides were unable to be separated and showed a single peak in chromatograms. In contrast, the polymer is hydrophobic and in the form of collapsed globule at 50°C. In this case, the peptides were completely separated because of the enhanced hydrophobic interaction of peptides and stationary phase.