Journal of Separation Science

Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Cover image for Vol. 35 Issue 22

Special Issue: Biotechnology

November 2012

Volume 35, Issue 22

Pages 3001–3234

Issue edited by: Michael Lämmerhofer

  1. Cover Picture

    1. Top of page
    2. Cover Picture
    3. Contents
    4. Editorial
    5. Reviews
    6. Media
    7. Metabolites, Lipids, and Bionanoparticles
    8. Proteins
    9. Nucleic Acids
    1. Cover Picture: J. Sep. Science 35'22

      Article first published online: 23 NOV 2012 | DOI: 10.1002/jssc.201270181

  2. Contents

    1. Top of page
    2. Cover Picture
    3. Contents
    4. Editorial
    5. Reviews
    6. Media
    7. Metabolites, Lipids, and Bionanoparticles
    8. Proteins
    9. Nucleic Acids
    1. Contents: J. Sep. Science 35'22

      Article first published online: 23 NOV 2012 | DOI: 10.1002/jssc.201270182

  3. Editorial

    1. Top of page
    2. Cover Picture
    3. Contents
    4. Editorial
    5. Reviews
    6. Media
    7. Metabolites, Lipids, and Bionanoparticles
    8. Proteins
    9. Nucleic Acids
    1. Separation Science: Enabling Technology in Biotech

      Article first published online: 23 NOV 2012 | DOI: 10.1002/jssc.201270183

  4. Reviews

    1. Top of page
    2. Cover Picture
    3. Contents
    4. Editorial
    5. Reviews
    6. Media
    7. Metabolites, Lipids, and Bionanoparticles
    8. Proteins
    9. Nucleic Acids
    1. You have free access to this content
      Methods for characterization of biochromatography media (pages 3001–3032)

      Rainer Hahn

      Article first published online: 30 OCT 2012 | DOI: 10.1002/jssc.201200770

    2. Fast separations of intact proteins by liquid chromatography (pages 3033–3045)

      Fei Wang, Yi Min and Xindu Geng

      Article first published online: 30 OCT 2012 | DOI: 10.1002/jssc.201200339

    3. Advances in chromatographic supports for pharmaceutical-grade plasmid DNA purification (pages 3046–3058)

      Ângela Sousa, Fani Sousa and João A. Queiroz

      Article first published online: 7 SEP 2012 | DOI: 10.1002/jssc.201200307

    4. Application of monoliths for bioparticle isolation (pages 3059–3072)

      Aleš Podgornik and Nika Lendero Krajnc

      Article first published online: 30 OCT 2012 | DOI: 10.1002/jssc.201200387

  5. Media

    1. Top of page
    2. Cover Picture
    3. Contents
    4. Editorial
    5. Reviews
    6. Media
    7. Metabolites, Lipids, and Bionanoparticles
    8. Proteins
    9. Nucleic Acids
    1. Molecular modeling of polymeric adsorbent media: The effects of counter-ions on ligand immobilization and pore structure (pages 3073–3083)

      Enrico Riccardi, Jee-Ching Wang and Athanasios I. Liapis

      Article first published online: 22 OCT 2012 | DOI: 10.1002/jssc.201200529

    2. Protein adsorption on DEAE ion-exchange resins with different ligand densities and pore sizes (pages 3084–3090)

      Lu Hui-Li, Lin Dong-Qiang, Zhu Mi-Mi and Yao Shan-Jing

      Article first published online: 18 JUN 2012 | DOI: 10.1002/jssc.201101083

  6. Metabolites, Lipids, and Bionanoparticles

    1. Top of page
    2. Cover Picture
    3. Contents
    4. Editorial
    5. Reviews
    6. Media
    7. Metabolites, Lipids, and Bionanoparticles
    8. Proteins
    9. Nucleic Acids
    1. U13C cell extract of Pichia pastoris – a powerful tool for evaluation of sample preparation in metabolomics (pages 3091–3105)

      Stefan Neubauer, Christina Haberhauer-Troyer, Kristaps Klavins, Hannes Russmayer, Matthias G. Steiger, Brigitte Gasser, Michael Sauer, Diethard Mattanovich, Stephan Hann and Gunda Koellensperger

      Article first published online: 22 OCT 2012 | DOI: 10.1002/jssc.201200447

    2. Comparison of lipid sinks in sequestering common intoxicating drugs (pages 3106–3112)

      Jana Lokajová, Juha M. Holopainen and Susanne K. Wiedmer

      Article first published online: 23 NOV 2012 | DOI: 10.1002/jssc.201101038

  7. Proteins

    1. Top of page
    2. Cover Picture
    3. Contents
    4. Editorial
    5. Reviews
    6. Media
    7. Metabolites, Lipids, and Bionanoparticles
    8. Proteins
    9. Nucleic Acids
    1. Impact of mobile phase temperature on recovery and stability of monoclonal antibodies using recent reversed-phase stationary phases (pages 3113–3123)

      Szabolcs Fekete, Serge Rudaz, Jean-Luc Veuthey and Davy Guillarme

      Article first published online: 20 JUN 2012 | DOI: 10.1002/jssc.201200297

    2. Robustness of iCIEF methodology for the analysis of monoclonal antibodies: An interlaboratory study (pages 3124–3129)

      Oscar Salas-Solano, Babu Kennel, SungAe Suhr Park, Kelly Roby, Zoran Sosic, Boris Boumajny, Sarah Free, Angelia Reed-Bogan, David Michels, Will McElroy, Pauline Bonasia, Mingfang Hong, Xiaoping He, Margaret Ruesch, Frank Moffatt, Steffen Kiessig and Brian Nunnally

      Article first published online: 15 OCT 2012 | DOI: 10.1002/jssc.201200633

    3. Mechanism of improved antibody aggregate separation in polyethylene glycol-modulated cation exchange chromatography (pages 3130–3138)

      Simon Kluters, Sebastian Neumann, Thomas von Hirschheydt, Adelbert Grossmann, Andreas Schaubmar and Christian Frech

      Article first published online: 30 OCT 2012 | DOI: 10.1002/jssc.201200715

    4. Purification of polyclonal antibodies from Cohn fraction II + III, skim milk, and whey by affinity chromatography using a hexamer peptide ligand (pages 3139–3148)

      Stefano Menegatti, Amith D. Naik, Patrick V. Gurgel and Ruben G. Carbonell

      Article first published online: 2 JUL 2012 | DOI: 10.1002/jssc.201200199

    5. Development and characterization of an automated high throughput screening method for optimization of protein refolding processes (pages 3149–3159)

      Annette Berg, Stefan A. Oelmeier, Jörg Kittelmann, Florian Dismer and Jürgen Hubbuch

      Article first published online: 23 JUL 2012 | DOI: 10.1002/jssc.201200306

    6. Optimization of a refolding step for a therapeutic fusion protein in the quality by design (QbD) paradigm (pages 3160–3169)

      Pratap D. Bade, Susmitha P. Kotu and Anurag S. Rathore

      Article first published online: 22 OCT 2012 | DOI: 10.1002/jssc.201200476

    7. Cation exchange displacement batch chromatography of proteins guided by screening of protein purification parameters (pages 3170–3176)

      Marta Kotasińska, Verena Richter, Joachim Thiemann and Hartmut Schlüter

      Article first published online: 18 JUN 2012 | DOI: 10.1002/jssc.201200329

    8. Application of a micromembrane chromatography module to the examination of protein adsorption equilibrium (pages 3177–3183)

      Natália Káňavová, Anna Kosior, Monika Antošová, René Faber and Milan Polakovič

      Article first published online: 21 AUG 2012 | DOI: 10.1002/jssc.201200396

    9. Purification of transthyretin as nutritional biomarker of selenium status (pages 3184–3189)

      Andrea Mahn, María Elena Lienqueo, Claudia Quilodrán and Alvaro Olivera-Nappa

      Article first published online: 4 OCT 2012 | DOI: 10.1002/jssc.201200646

  8. Nucleic Acids

    1. Top of page
    2. Cover Picture
    3. Contents
    4. Editorial
    5. Reviews
    6. Media
    7. Metabolites, Lipids, and Bionanoparticles
    8. Proteins
    9. Nucleic Acids
    1. Validation and scale-up of plasmid DNA purification by phenyl-boronic acid chromatography (pages 3190–3196)

      A. Gabriela Gomes, Ana M. Azevedo, M. Raquel Aires-Barros and D. Miguel F. Prazeres

      Article first published online: 23 NOV 2012 | DOI: 10.1002/jssc.201200225

    2. High-throughput screening-based selection and scale-up of aqueous two-phase systems for pDNA purification (pages 3197–3207)

      Matthias Wiendahl, Stefan A. Oelmeier, Florian Dismer and Jürgen Hubbuch

      Article first published online: 9 AUG 2012 | DOI: 10.1002/jssc.201200310

    3. Removal of endotoxins from plasmid DNA: Analysis of aggregative interaction of mobile divalent metal cations with endotoxins and plasmid DNA (pages 3208–3216)

      Clarence M. Ongkudon, Emma Hodges, Kathleen Murphy and Michael K. Danquah

      Article first published online: 24 SEP 2012 | DOI: 10.1002/jssc.201200237

    4. A new strategy for RNA isolation from eukaryotic cells using arginine affinity chromatography (pages 3217–3226)

      Rita Martins, Cláudio J. Maia, João A. Queiroz and Fani Sousa

      Article first published online: 24 SEP 2012 | DOI: 10.1002/jssc.201200338

    5. Toward reading the sequence of short oligonucleotides from their retention factors obtained by means of hydrophilic interaction chromatography and ion-interaction reversed-phase liquid chromatography (pages 3227–3234)

      Miroslava Bittová, Jan Havliš, Hana Fuksová, Blanka Vrbková and Libuše Trnková

      Article first published online: 23 NOV 2012 | DOI: 10.1002/jssc.201200482

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