Journal of Separation Science

Cover image for Vol. 38 Issue 7

April 2015

Volume 38, Issue 7

Pages vii–viii, 1073–1261

  1. Front Cover

    1. Top of page
    2. Front Cover
    3. Back Cover
    4. Editorial Board
    5. Contents
    6. Editorial
    7. Liquid Chromatography
    8. Sample Preparation
    9. Electro Driven Separations
    10. Other Techniques
    1. An ultra high performance liquid chromatography with tandem mass spectrometry method for plasma and cerebrospinal fluid pharmacokinetics of rhein in patients with traumatic brain injury after administration of rhubarb decoction

      Yang Wang, Rong Fan, Jiekun Luo, Tao Tang, Zhihua Xing, Zian Xia, Weijun Peng, Wenzhu Wang, Huiying Lv, Wei Huang, Yizeng Liang, Lunzhao Yi, Hongmei Lu and Xi Huang

      Article first published online: 17 APR 2015 | DOI: 10.1002/jssc.201570071

      Thumbnail image of graphical abstract

      (J. Sep. Sci. 2015, 38, 1100–1108)

      Damage of the blood-brain barrier is a common result of traumatic brain injury. This damage can open the blood-brain barrier and allow drug passage. An ultra-performance liquid chromatography–tandem mass spectrometry method was established to determine the concentration of rhein in the biofluids (plasma and cerebrospinal fluid) of patients with a compromised blood-brain barrier following traumatic brain injury after rhubarb administration. Furthermore, the pharmacokinetic profiles were analyzed. A triple-quadruple tandem mass spectrometer with electrospray ionization was used for rhein detection. The mass transition followed was m/z 283.06[RIGHTWARDS ARROW]239.0. The calibration curve was linear in the concentration range of 10-4000 ng/mL for the biofluids. The intra- and inter-day precisions were less than 10%. The Relative Standard Deviation of recovery was less than 15% in biological matrices. The pharmacokinetic data showed that rhein was rapidly transported into biofluids, and exhibited a peak concentration 1 h after administration. The elimination rate of rhein was slow. The AUCcerebrospinal fluid/AUCplasma of rhein was approximately 17%, indicating that portions of rhein could pass the impaired blood-brain barrier. The method was successfully applied to quantify rhein in the biofluids of all patients. The data presented can help to guide clinical applications of rhubarb for treating traumatic brain injury.

  2. Back Cover

    1. Top of page
    2. Front Cover
    3. Back Cover
    4. Editorial Board
    5. Contents
    6. Editorial
    7. Liquid Chromatography
    8. Sample Preparation
    9. Electro Driven Separations
    10. Other Techniques
    1. Ultra high performance liquid chromatography with synapt high-definition mass spectrometry and a pattern recognition approach to characterize chemical constituents and rat metabolites after the oral administration of Phellinus igniarius

      Yu Dong, Huiyu Wang, Yang Zhang, Na An, Yue Zhang and Dan Shou

      Article first published online: 17 APR 2015 | DOI: 10.1002/jssc.201570072

      Thumbnail image of graphical abstract

      (J. Sep. Sci. 2015, 38, 1137–1148)

      Traditional Chinese medicine (TCM) is an essential part of the healthcare system in most Asian countries. For example, Phellinus igniarius is a popular medicinal mushroom in east Asia. Rapid, sensitive, and specific analytical methods for profiling and characterizing the chemical constituents of TCM, both in vitro and in vivo, are necessary and valuable. Serum pharmacochemistry methods have been shown to be useful for elucidating the effective constituents and metabolites in TCM. In this study the ultra-performance liquid chromatography synapt high-definition mass spectrometry, combined with principal component analysis, orthogonal partial least squared discriminant analysis, and MetabolynxTM software, is used to determine the chemical constituents and metabolites produced after oral administration of ethanol extract of Phellinus igniarius (EEPI) to understand the pharmacological effects of EEPI. There were 24 EEPI peaks within 10 min of analysis time and 20 of these were identified or tentatively characterized based on their fragmentation behaviors. In the S-plot of orthogonal partial least squared discriminant analysis, 27 ions were extracted to form the serum. Among them, 9 absorbed prototype components of EEPI and 18 metabolites were identified in vivo. Glucuronidation, oxidation and methylation were the major metabolic reactions found in EEPI. This is the first systematic analysis and characterization of the chemical constituents and metabolites in EEPI. This method can be applied to the analysis and characterization of constituents and metabolites of other material used in TCMs.

  3. Editorial Board

    1. Top of page
    2. Front Cover
    3. Back Cover
    4. Editorial Board
    5. Contents
    6. Editorial
    7. Liquid Chromatography
    8. Sample Preparation
    9. Electro Driven Separations
    10. Other Techniques
    1. Contents: J. Sep. Science 7'15

      Article first published online: 17 APR 2015 | DOI: 10.1002/jssc.201570073

  4. Contents

    1. Top of page
    2. Front Cover
    3. Back Cover
    4. Editorial Board
    5. Contents
    6. Editorial
    7. Liquid Chromatography
    8. Sample Preparation
    9. Electro Driven Separations
    10. Other Techniques
    1. Contents: J. Sep. Science 7'15

      Article first published online: 17 APR 2015 | DOI: 10.1002/jssc.201570074

  5. Editorial

    1. Top of page
    2. Front Cover
    3. Back Cover
    4. Editorial Board
    5. Contents
    6. Editorial
    7. Liquid Chromatography
    8. Sample Preparation
    9. Electro Driven Separations
    10. Other Techniques
  6. Liquid Chromatography

    1. Top of page
    2. Front Cover
    3. Back Cover
    4. Editorial Board
    5. Contents
    6. Editorial
    7. Liquid Chromatography
    8. Sample Preparation
    9. Electro Driven Separations
    10. Other Techniques
    1. An ultra high performance liquid chromatography with tandem mass spectrometry method for plasma and cerebrospinal fluid pharmacokinetics of rhein in patients with traumatic brain injury after administration of rhubarb decoction (pages 1100–1108)

      Yang Wang, Rong Fan, Jiekun Luo, Tao Tang, Zhihua Xing, Zian Xia, Weijun Peng, Wenzhu Wang, Huiying Lv, Wei Huang, Yizeng Liang, Lunzhao Yi, Hongmei Lu and Xi Huang

      Article first published online: 2 MAR 2015 | DOI: 10.1002/jssc.201401197

    2. Design of a microfluidic device for comprehensive spatial two-dimensional liquid chromatography (pages 1123–1129)

      Bert Wouters, Jelle De Vos, Gert Desmet, Herman Terryn, Peter J. Schoenmakers and Sebastiaan Eeltink

      Article first published online: 9 MAR 2015 | DOI: 10.1002/jssc.201401192

  7. Sample Preparation

    1. Top of page
    2. Front Cover
    3. Back Cover
    4. Editorial Board
    5. Contents
    6. Editorial
    7. Liquid Chromatography
    8. Sample Preparation
    9. Electro Driven Separations
    10. Other Techniques
    1. Ultrasound-assisted aqueous two-phase extraction of phenylethanoid glycosides from Cistanche deserticola Y. C. Ma stems (pages 1194–1203)

      Beitao Dong, Xiaofan Yuan, Qingsheng Zhao, Qingming Feng, Boyan Liu, Yuanheng Guo and Bing Zhao

      Article first published online: 26 FEB 2015 | DOI: 10.1002/jssc.201401410

    2. Development of a selective sorbent for the solid-phase extraction of terbuthylazine in olive oil samples: A molecular imprinting strategy (pages 1204–1212)

      Raquel Garcia, Nuno Martins, Elisabete P. Carreiro, Marco Simões, Maria M. L. Ribeiro Carrott, Peter J. M. Carrott, Anthony J. Burke and Maria João Cabrita

      Article first published online: 10 MAR 2015 | DOI: 10.1002/jssc.201401263

  8. Electro Driven Separations

    1. Top of page
    2. Front Cover
    3. Back Cover
    4. Editorial Board
    5. Contents
    6. Editorial
    7. Liquid Chromatography
    8. Sample Preparation
    9. Electro Driven Separations
    10. Other Techniques
    1. Supercritical fluid chromatography with photodiode array detection for pesticide analysis in papaya and avocado samples (pages 1240–1247)

      Norma S. Pano-Farias, Silvia G. Ceballos-Magaña, Jorge Gonzalez, José M. Jurado and Roberto Muñiz-Valencia

      Article first published online: 14 FEB 2015 | DOI: 10.1002/jssc.201401174

  9. Other Techniques

    1. Top of page
    2. Front Cover
    3. Back Cover
    4. Editorial Board
    5. Contents
    6. Editorial
    7. Liquid Chromatography
    8. Sample Preparation
    9. Electro Driven Separations
    10. Other Techniques
    1. Preparation of 17β-estradiol-imprinted material by surface-initiated atom transfer radical polymerization and its application (pages 1254–1261)

      Yanru Gong, Yuling Niu, Xiaohan Gong, Meihua Ma, Xiaowei Ren, Weihua Zhu, Ruiming Luo and Bolin Gong

      Article first published online: 27 FEB 2015 | DOI: 10.1002/jssc.201401375

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