Original Paper

Photostimulation of two types of Ca2+ waves in rat pheochromocytoma PC12 cells by ultrashort pulsed near-infrared laser irradiation

  1. N. I. Smith,
  2. S. Iwanaga2,
  3. T. Beppu2,
  4. K. Fujita2,
  5. O. Nakamura1,
  6. S. Kawata2

Article first published online: 13 OCT 2005

DOI: 10.1002/lapl.200510067

Issue

Laser Physics Letters

Laser Physics Letters

Volume 3, Issue 3, pages 154–161, March 2006

Additional Information

How to Cite

Smith, N. I., Iwanaga, S., Beppu, T., Fujita, K., Nakamura, O. and Kawata, S. (2006), Photostimulation of two types of Ca2+ waves in rat pheochromocytoma PC12 cells by ultrashort pulsed near-infrared laser irradiation. Laser Physics Letters, 3: 154–161. doi: 10.1002/lapl.200510067

Author Information

  1. 1

    Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University, Suita, Yamada-oka 1-3, Osaka-fu, 565-0871, Japan

  2. 2

    Department of Applied Physics, School of Engineering, Osaka University, Suita, Yamada-oka 2-1, Osaka-fu, 565-0871, Japan

Publication History

  1. Issue published online: 1 FEB 2006
  2. Article first published online: 13 OCT 2005
  3. Manuscript Accepted: 3 OCT 2005
  4. Manuscript Received: 30 SEP 2005

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Keywords:

  • photostimulation;
  • calcium wave;
  • rat pheochromocytoma PC12 neurons;
  • ultrashort pulsed laser

Abstract

We show that focused laser irradiation can generate Ca2+ waves in differentiated PC12 cells, using highly penetrating and locally acting near-infrared femtosecond pulsed laser light. Similar to photolytic uncaging experiments, intracellular Ca2+ concentrations could be elevated to produce a whole cell Ca2+ rise, but without the requirement for caged Ca2+. Irradiation of subcellular volumes in the soma or in PC12 neurites generated a relatively slow calcium rise (speed ∼20 µm/s). Less frequently, a fast calcium rise (speed >1200 µm/s) was observed, consistent with laser-induced membrane depolarization. The probability of Ca2+ generation increased with laser power and was found to be the same in excitable (PC12) and nonexcitable (HeLa) cells. (© 2006 by Astro, Ltd. Published exclusively by WILEY-VCH Verlag GmbH & Co. KGaA)

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