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Laser Methods in Chemistry, Biology, and Medicine
A new benchmark for time-resolved detection of singlet oxygen luminescence – revealing the evolution of lifetime in living cells with low dose illumination
Article first published online: 4 NOV 2008
DOI: 10.1002/lapl.200810116
Copyright © 2009 by Astro Ltd., published exclusively by WILEY-VCH Verlag GmbH & Co. KGaA
1612-202X/asset/cover.gif?v=1&s=4cbf107bd52f59b66eeea9cadbff11b4c77793fc "Laser Physics Letters")
Additional Information
How to Cite
Schlothauer, J., Hackbarth, S. and Röder, B. (2009), A new benchmark for time-resolved detection of singlet oxygen luminescence – revealing the evolution of lifetime in living cells with low dose illumination. Laser Physics Letters, 6: 216–221. doi: 10.1002/lapl.200810116
Publication History
- Issue published online: 2 FEB 2009
- Article first published online: 4 NOV 2008
- Manuscript Accepted: 20 OCT 2008
- Manuscript Revised: 17 OCT 2008
- Manuscript Received: 15 OCT 2008
Funded by
- German Ministry of Education and Science (BMBF)
- Abstract
- References
- Cited By
Keywords:
- singlet oxygen;
- photo physics;
- photo medicine;
- cell;
- photodynamic therapy;
- photosensitizer;
- quencher;
- phototoxicity;
- luminescence;
- phosphorescence;
- time correlated single photon counting;
- TCSPC;
- time correlated multi photon counting;
- TCMPC
Abstract
The presented setup for singlet oxygen luminescence detection in cell suspensions outperforms the current setups by more than one order-of-magnitude gain in sensitivity. This not only allows precise determination of the singlet oxygen lifetime in cells but also reveals a previously unnoticeable behaviour of the singlet oxygen luminescence during low dose illumination. (© 2009 by Astro Ltd., Published exclusively by WILEY-VCH Verlag GmbH & Co. KGaA)