Towards multimodal nonlinear optical tomography – experimental methodology

Authors

  • N. Vogler,

    1. Institute for Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University, Helmholtzweg 4, 07743 Jena, Germany
    2. Institute for Photonic Technology Jena IPHT, Albert-Einstein-Str. 9, 07745 Jena, Germany
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  • A. Medyukhina,

    1. Institute for Photonic Technology Jena IPHT, Albert-Einstein-Str. 9, 07745 Jena, Germany
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  • I. Latka,

    1. Institute for Photonic Technology Jena IPHT, Albert-Einstein-Str. 9, 07745 Jena, Germany
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  • S. Kemper,

    1. Department of Dermatology, University Münster, Schlossplatz 2, 48149 Münster, Germany
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  • M. Böhm,

    1. Department of Dermatology, University Münster, Schlossplatz 2, 48149 Münster, Germany
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  • B. Dietzek,

    1. Institute for Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University, Helmholtzweg 4, 07743 Jena, Germany
    2. Institute for Photonic Technology Jena IPHT, Albert-Einstein-Str. 9, 07745 Jena, Germany
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  • J. Popp

    Corresponding author
    1. Institute for Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University, Helmholtzweg 4, 07743 Jena, Germany
    2. Institute for Photonic Technology Jena IPHT, Albert-Einstein-Str. 9, 07745 Jena, Germany
    • Institute for Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University, Helmholtzweg 4, 07743 Jena, Germany
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Abstract

All-optical microspectroscopic and tomographic tools reveal great potential for clinical dermatologic diagnostics, i.e., investigation of human skin and skin diseases. While optical-coherence tomography has been complemented by two-photon fluorescence tomography and second-harmonic generation tomography, a joint study of various nonlinear optical microspectroscopies, i.e., application of the recently developed multimodal imaging approach, to sizable human-tissue samples has not been evaluated up to now. Here, we present such multi-modal approach combining different nonlinear optical contrast mechanisms for imaging, namely two-photon excited fluorescence (TPF), second-harmonic generation (SHG), and coherent anti-Stokes Raman scattering (CARS) into a joint microscopic experiment. We show the potential of imaging large skin areas and discuss the information obtained in a case study comparing normal skin and keloid tissue. (© 2011 by Astro Ltd., Published exclusively by WILEY-VCH Verlag GmbH & Co. KGaA) (© 2011 by Astro Ltd., Published exclusively by WILEY-VCH Verlag GmbH & Co. KGaA)

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