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Keywords:

  • Transforming growth factor-β;
  • osteoinduction;
  • cytokines

Abstract

Objective:

To determine if there is a dose–response curve for TGF-β1 in a rabbit calvarial defect model.

Study Design:

Controlled animal study using arms of increasing concentrations of TGF-β1 to evaluate the osteoinductive potential of each arm.

Methods:

Sixteen skeletally mature New Zealand white rabbits were randomized into control and experimental arms. Incremental doses of TGF-β1 delivered in an inactivated guanidine-extracted demineralized bone matrix (Gu-DBM) carrier were implanted into a critically sized calvarial defect. The animals were sacrificed at 4 weeks and histomorphometric analysis was then accomplished.

Results:

TGF-β1 showed a dose–response relationship, with the higher doses chosen for this study causing more robust osteoinduction.

Conclusions:

In this pilot dosing study, TGF-β1 demonstrates increasing osteoinduction with increasing dose levels in this animal model. Laryngoscope, 119:126–130, 2009