IgE response to staphylococcal enterotoxins in adenoid tissues from atopic children

Authors

  • Seung-Youp Shin MD,

    1. Department of Otolaryngology, Kyunghee University Hospital and Kyunghee University College of Medicine, Seoul, Korea
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  • Gil-Soon Choi MD,

    1. Department of Allergy and Rheumatology, Ajou University Hospital and Ajou University School of Medicine, Suwon, Korea
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  • Kun-Hee Lee MD, PhD,

    1. Department of Otolaryngology, Kyunghee University Hospital and Kyunghee University College of Medicine, Seoul, Korea
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  • Sung-Wan Kim MD, PhD,

    1. Department of Otolaryngology, Kyunghee University Hospital and Kyunghee University College of Medicine, Seoul, Korea
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  • Joong-Saeng Cho MD, PhD,

    1. Department of Otolaryngology, Kyunghee University Hospital and Kyunghee University College of Medicine, Seoul, Korea
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  • Hae-Sim Park MD, PhD

    Corresponding author
    1. Department of Allergy and Rheumatology, Ajou University Hospital and Ajou University School of Medicine, Suwon, Korea
    • Department of Allergy and Rheumatology, Ajou University, School of Medicine, Wonchondong San-5, Paldalgu, Suwon 442-749, Korea
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Abstract

Objectives/Hypothesis:

To evaluate local production of staphylococcal superantigen (SAg)-specific IgE in adenoid tissue and to compare its prevalence with that in the tonsil and serum, as well as its relationship with markers of allergic inflammation within adenoid tissue.

Study design:

Prospective randomized study.

Methods:

We recruited 18 atopic children who had rhinitis symptoms and were sensitized to more than one common aeroallergen, and 22 nonatopic children undergoing adenotonsillectomy. Immunoassays were performed using adenoid tissue homogenate to quantify the levels of three staphylococcal SAg-specific IgE (SEA, SEB, and TSST-1), total IgE, eosinophil cationic protein (ECP), mast cell tryptase, and soluble CD23.

Results:

Three kinds of SAg-specific IgE were detected in the adenoid and tonsil tissues of atopic patients, but not in those of nonatopic patients. In atopic children, the prevalence of SEA-, SEB-, and TSST-1-specific IgE in adenoid tissues (61.1%, 27.8%, 33.3%, respectively) were higher than those in tonsil tissues (38.9%, 5.6%, 11.1%, respectively) and in sera (11.1%, 27.8%, 16.7%, respectively). Subjects with high SEA levels showed significantly higher serum and adenoid total IgE, with higher eosinophilia. Significant correlations were noted between SAg-specific IgE levels and tryptase levels in adenoid tissue.

Conclusions:

Local specific IgE response to staphylococcal SAgs, especially SEA may contribute to ongoing allergic inflammation in adenoid tissue from atopic children. Laryngoscope, 119:171–175, 2009

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