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Somatostatin and gentamicin-induced auditory hair cell loss

Authors

  • Antje Caelers PhD,

    1. Department of Biomedicine University Hospital Basel and the Klinik für Ohren-, Nasen-, Halskrankheiten University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland
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  • Arianne Monge MD,

    1. Department of Biomedicine University Hospital Basel and the Klinik für Ohren-, Nasen-, Halskrankheiten University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland
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  • Yves Brand MD,

    1. Department of Biomedicine University Hospital Basel and the Klinik für Ohren-, Nasen-, Halskrankheiten University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland
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  • Daniel Bodmer MD, PhD

    Corresponding author
    1. Department of Biomedicine University Hospital Basel and the Klinik für Ohren-, Nasen-, Halskrankheiten University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland
    • Klinik für Ohren-, Nasen-, Halskrankheiten University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland
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Abstract

Objective/Hypothesis:

Hair cells of the mammalian auditory system do not regenerate, and therefore their loss leads to irreversible hearing loss. Aminoglycosides, among other substances, can irreversibly damage hair cells. Somatostatin, a peptide with hormone/neurotransmitter properties, has neuroprotective effects by binding to its receptor. In this study, we tested whether somatostatin can protect hair cells from gentamicin-induced damage in vitro.

Study Design:

This study confirmed the expression of somatostatin receptor mRNA within the cochlea and analyzed the effect of somatostatin on gentamicin-induced hair cell damage and death in vitro.

Methods:

Expression of somatostatin receptor mRNA in the rat cochlea was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR). Protection of auditory hair cells from gentamicin was tested using two different concentrations (1 μM and 5 μM, respectively) of somatostatin.

Results:

We detected somatostatin receptor-1 and -2 mRNA and in the organ of Corti (OC), spiral ganglion, and stria vascularis by RT-PCR. Moreover, we could see significantly less hair cell loss in the OCs that were pretreated with either 1 μM or 5 μM of somatostatin as compared with samples treated with gentamicin alone.

Conclusions:

Decreased hair cell loss in somatostatin-treated samples that had been exposed to gentamicin provides evidence for a protective effect of somatostatin in aminoglycoside-induced hair cell death in vitro.

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