Presented at the Triological Society Annual Meeting at COSM, Orlando, Florida, U.S.A., May 3, 2008.
Article first published online: 4 MAR 2009
Copyright © 2009 The American Laryngological, Rhinological, and Otological Society, Inc.
Volume 119, Issue 4, pages 799–805, April 2009
How to Cite
Kumai, Y., Kobler, J. B., Park, H., Lopez-Guerra, G., Karajanagi, S., Herrera, V. L.M. and Zeitels, S. M. (2009), Crosstalk between adipose-derived stem/stromal cells and vocal fold fibroblasts in vitro. The Laryngoscope, 119: 799–805. doi: 10.1002/lary.20149
This work was supported in part by the Eugene B. Casey Foundations and the Institute of Laryngology and Voice Restoration.
- Issue published online: 25 MAR 2009
- Article first published online: 4 MAR 2009
- Manuscript Accepted: 7 OCT 2008
- Adipose-derived stem cells;
- vocal fold scar;
To explore adipose-derived stem cell/fibroblast interactions as a potential remodeling pathway for vocal fold scar.
Fibroblasts and adipose-derived stem/stromal cells (ASCs) were cultured alone and in combination in a cell-contact–independent paracrine system. Analyses of cell proliferation, and the production of hyaluronic acid (HA) and collagen were performed on samples collected on days 1, 3, and 7.
Normal fibroblasts (NFs) were isolated bilaterally from the subepithelial lamina propria of two normal ferret vocal folds. Scar fibroblasts (SFs) were isolated from vocal folds that were electrocauterized 2 weeks before harvest. ASCs were isolated from lipoaspirated subcutaneous abdominal fat of two ferrets. A transwell cell-contact–independent cell communication culturing system was used for coculture experiments. Cells were seeded at 50,000/well in both monoculture and coculture experiments.
In monoculture, SFs proliferated faster and produced less HA and more collagen than NFs at day 7 (P < .05). In SF/ASC coculture, SF proliferation was diminished and collagen production at day 7 decreased (P < .05). HA production did not differ between monoculture and coculture conditions.
Normal and scar-tissue–derived vocal fold fibroblasts maintain phenotypic differences in culture, thus validating this in vitro scar model. In co-culture, contact-independent crosstalk occurs between SFs and ASCa, leading to less collagen secretion. The data support the hypothesis that ASCs can induce favorable remodeling of scarred vocal folds in vivo by their interactions with endogenous fibroblasts. Laryngoscope, 2009