Characterization of raised phonation in an evoked rabbit phonation model

Authors

  • Erik R. Swanson MD,

    1. Department of Otolaryngology, Vanderbilt University Bill Wilkerson Center for Otolaryngology and Communication Sciences, Nashville, Tennessee, U.S.A.
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  • Davood Abdollahian BS,

    1. Department of Otolaryngology, Vanderbilt University Bill Wilkerson Center for Otolaryngology and Communication Sciences, Nashville, Tennessee, U.S.A.
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  • Tsunehisa Ohno MD,

    1. Department of Otolaryngology, Vanderbilt University Bill Wilkerson Center for Otolaryngology and Communication Sciences, Nashville, Tennessee, U.S.A.
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  • Pingjiang Ge MD,

    1. Department of Otolaryngology, Vanderbilt University Bill Wilkerson Center for Otolaryngology and Communication Sciences, Nashville, Tennessee, U.S.A.
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  • David L. Zealear PhD,

    1. Department of Otolaryngology, Vanderbilt University Bill Wilkerson Center for Otolaryngology and Communication Sciences, Nashville, Tennessee, U.S.A.
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  • Bernard Rousseau PhD

    Corresponding author
    1. Department of Otolaryngology, Vanderbilt University Bill Wilkerson Center for Otolaryngology and Communication Sciences, Nashville, Tennessee, U.S.A.
    • Vanderbilt University, Department of Otolaryngology, 1313 21st Avenue South, Room 602, Nashville, TN 37232-4480
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  • To be presented at the 130th Annual Meeting of the American Laryngological Association, Phoenix, Arizona, U.S.A., May 28–29, 2009.

  • This work was supported by NIH grant R03 DC 008400 from the National Institute of Deafness and Other Communication Disorders (NIDCD).

Abstract

Objectives/Hypothesis:

Our laboratory has developed an in vivo rabbit model to investigate the effects of phonation on expression and turnover of the vocal fold extracellular matrix. As a logical outgrowth of this research to include phonotrauma in the present study, we investigated the hypothesis that an increase in airflow rate delivered to the glottis produces a change in glottal configuration and an increase in mean phonation intensity.

Study Design:

Prospective animal study.

Methods:

Six New Zealand white breeder rabbits weighing 3 to 5 kg were used in this study. A rigid endoscope and camera were used to document glottal configuration. Acoustic signals of modal and raised phonation were recorded and digitized. Two separate one-way repeated measures analysis of variance (ANOVA) tests were used to investigate within subject differences in phonation intensity and fundamental frequency between modal and raised phonation.

Results:

Phonation intensity was 54.19 dB SPL (6.21 standard deviations [SD]) during modal phonation, and 60.31 dB SPL (5.68 SD) during raised phonation. Endoscopic images revealed a convergent glottis, with greater separation of the vocal folds during raised phonation. Results of ANOVA revealed a significant within subjects effect for phonation intensity (P = .011). Pairwise comparisons revealed that phonation intensity increased significantly during raised phonation, compared to modal phonation (P = .008). No differences in mean fundamental frequency were observed between phonation conditions.

Conclusions:

Improved understanding of factors that control phonation output in the in vivo rabbit model will result in improved capabilities to match phonation dose across animals and provide immediate direction to future biochemical studies. Laryngoscope, 2009

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