Avi Khafif, MD and Shahar Lev-Ari, PhD contributed equally to this work. The authors have no conflicts of interest to disclose.
Curcumin: A potential radio-enhancer in head and neck cancer†
Version of Record online: 4 AUG 2009
Copyright © 2009 The American Laryngological, Rhinological, and Otological Society, Inc.
Volume 119, Issue 10, pages 2019–2026, October 2009
How to Cite
Khafif, A., Lev-Ari, S., Vexler, A., Barnea, I., Starr, A., Karaush, V., Haif, S. and Ben-Yosef, R. (2009), Curcumin: A potential radio-enhancer in head and neck cancer. The Laryngoscope, 119: 2019–2026. doi: 10.1002/lary.20582
- Issue online: 22 SEP 2009
- Version of Record online: 4 AUG 2009
- Manuscript Accepted: 13 MAY 2009
- Head and neck squamous cell carcinoma;
- epithelial growth factor receptor
To investigate whether curcumin enhances the cytotoxic effect of radiotherapy in head and neck squamous cell carcinoma (HNSCC).
HNSCC cell lines SCC-1, SCC-9, KB, as well as A431 cell line were treated with curcumin, irradiation, or their combination. Cell viability was evaluated by XTT assay. Cyclooxygenase-2 (COX-2), epithelial growth factor receptor (EGFR), and p-Erk1/2 were measured by Western blot analysis. CD-1 athymic nude mice with orthotopic implanted SCC-1 cells, were treated with control diet, curcumin containing diet, local single-dose radiation, or combination.
Curcumin (IC50 range, 15–22 μM) and radiation inhibited cell viability in all cell lines were tested. The combination of curcumin and radiation resulted in additive effect. Curcumin decreased COX-2 expression and inhibited phosphorylation of EGFR in SCC-1 cells. In tumor-bearing mice the combination regimen showed a decrease in both tumor weight (25%, P = .09) and tumor size (15%, P = .23) compared to the nontreated mice.
Curcumin inhibited HNSCC cell growth and augmented the effect of radiation in vitro and in vivo. A possible mechanism is inhibition of COX-2 expression and EGFR phosphorylation. Laryngoscope, 2009