Summer Hanson, MD, Jaehyup Kim, MD, and Beatriz H. Quinchia Johnson, DDS, PhD contributed equally to this work.
Characterization of mesenchymal stem cells from human vocal fold fibroblasts†
Article first published online: 3 FEB 2010
Copyright © 2010 The American Laryngological, Rhinological, and Otological Society, Inc.
Volume 120, Issue 3, pages 546–551, March 2010
How to Cite
Hanson, S. E., Kim, J., Johnson, B. H. Q., Bradley, B., Breunig, M. J., Hematti, P. and Thibeault, S. L. (2010), Characterization of mesenchymal stem cells from human vocal fold fibroblasts. The Laryngoscope, 120: 546–551. doi: 10.1002/lary.20797
This work was supported in part by NIH/NIDCD Grant R01 DC4336 (S. L. Thibeault), NIH/NHLBI Grant HL081076 K08 (P. Hematti), and NIH T32 Physician-Scientist Training Grant CA009614 (S. E. Hanson).
- Issue published online: 16 FEB 2010
- Article first published online: 3 FEB 2010
- Manuscript Accepted: 3 NOV 2009
- NIH/NIDCD Grant. Grant Number: R01 DC4336
- NIH/NHLBI Grant. Grant Number: HL081076 K08
- NIH T32 Physician-Scientist Training Grant. Grant Number: CA009614
- Mesenchymal stem cells;
- human vocal fold stem cells;
- immune modulation;
- vocal fold fibroblasts
Mesenchymal stem cells (MSCs) originally isolated from bone marrow (BM), are fibroblast-looking cells that are now assumed to be present in the stromal component of many tissues. MSCs are characterized by a certain set of criteria, including their growth culture characteristics, a combination of cell surface markers, and the ability to differentiate along multiple mesenchymal tissue lineages. We hypothesized that human vocal fold fibroblasts (hVFF) isolated from the lamina propria meet the criteria established to define MSCs and are functionally similar to MSCs derived from BM and adipose tissue.
In vitro study.
hVFF were previously derived from human vocal fold tissues. MSCs were derived from adipose tissue (AT), and BM of healthy donors based on their attachment to culture dishes and their morphology and expanded in culture. Cells were analyzed for standard cell surface markers identified on BM-derived MSCs and the ability to differentiate into cells of mesenchymal lineage (i.e., fat, bone, and cartilage). We investigated the immunophenotype of these cells before and after interferon-γ (INF-γ) stimulation.
hVFF displayed cell surface markers and multipotent differentiation capacity characteristic of MSCs. Furthermore, these cells exhibited similar patterns of expression of human leukocyte antigen and costimulatory molecules, after stimulation with INF-γ compared to MSCs derived from BM and AT.
Based on our findings, hVFF derived from lamina propria have the same cell surface markers, immunophenotypic characteristics, and differentiation potential as BM- and AT-derived MSCs. We propose that vocal fold fibroblasts are MSCs resident in the vocal fold lamina propria. Laryngoscope, 2010