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Characterization of mesenchymal stem cells from human vocal fold fibroblasts

Authors

  • Summer E. Hanson MD,

    1. Division of Plastic and Reconstructive Surgery, School of Medicine and Public Health, University of Wisconsin–Madison, Madison, Wisconsin, U.S.A
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    • Summer Hanson, MD, Jaehyup Kim, MD, and Beatriz H. Quinchia Johnson, DDS, PhD contributed equally to this work.

  • Jaehyup Kim MD,

    1. Department of Medicine, School of Medicine and Public Health, University of Wisconsin–Madison, Madison, Wisconsin, U.S.A
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    • Summer Hanson, MD, Jaehyup Kim, MD, and Beatriz H. Quinchia Johnson, DDS, PhD contributed equally to this work.

  • Beatriz H. Quinchia Johnson DDS, PhD,

    1. Division of Otolaryngology–Head and Neck Surgery, University of Wisconsin–Madison, Madison, Wisconsin, U.S.A
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    • Summer Hanson, MD, Jaehyup Kim, MD, and Beatriz H. Quinchia Johnson, DDS, PhD contributed equally to this work.

  • Bridget Bradley,

    1. College of Letters and Sciences, University of Wisconsin–Madison, Wisconsin, U.S.A
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  • Melissa J. Breunig,

    1. College of Agriculture and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin, U.S.A
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  • Peiman Hematti MD,

    Corresponding author
    1. Department of Medicine, School of Medicine and Public Health, University of Wisconsin–Madison, Madison, Wisconsin, U.S.A
    2. University of Wisconsin Carbone Cancer Center, Madison, Wisconsin, U.S.A
    • Otolaryngology Office Hematology Office, WIMR 5107 WIMR 4033, 1111 Highland Avenue, Madison, WI 53705-2725
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  • Susan L. Thibeault PhD

    Corresponding author
    1. Division of Otolaryngology–Head and Neck Surgery, University of Wisconsin–Madison, Madison, Wisconsin, U.S.A
    2. University of Wisconsin Carbone Cancer Center, Madison, Wisconsin, U.S.A
    • Otolaryngology Office Hematology Office, WIMR 5107 WIMR 4033, 1111 Highland Avenue, Madison, WI 53705-2725
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  • This work was supported in part by NIH/NIDCD Grant R01 DC4336 (S. L. Thibeault), NIH/NHLBI Grant HL081076 K08 (P. Hematti), and NIH T32 Physician-Scientist Training Grant CA009614 (S. E. Hanson).

Abstract

Objectives/Hypothesis:

Mesenchymal stem cells (MSCs) originally isolated from bone marrow (BM), are fibroblast-looking cells that are now assumed to be present in the stromal component of many tissues. MSCs are characterized by a certain set of criteria, including their growth culture characteristics, a combination of cell surface markers, and the ability to differentiate along multiple mesenchymal tissue lineages. We hypothesized that human vocal fold fibroblasts (hVFF) isolated from the lamina propria meet the criteria established to define MSCs and are functionally similar to MSCs derived from BM and adipose tissue.

Study Design:

In vitro study.

Methods:

hVFF were previously derived from human vocal fold tissues. MSCs were derived from adipose tissue (AT), and BM of healthy donors based on their attachment to culture dishes and their morphology and expanded in culture. Cells were analyzed for standard cell surface markers identified on BM-derived MSCs and the ability to differentiate into cells of mesenchymal lineage (i.e., fat, bone, and cartilage). We investigated the immunophenotype of these cells before and after interferon-γ (INF-γ) stimulation.

Results:

hVFF displayed cell surface markers and multipotent differentiation capacity characteristic of MSCs. Furthermore, these cells exhibited similar patterns of expression of human leukocyte antigen and costimulatory molecules, after stimulation with INF-γ compared to MSCs derived from BM and AT.

Conclusions:

Based on our findings, hVFF derived from lamina propria have the same cell surface markers, immunophenotypic characteristics, and differentiation potential as BM- and AT-derived MSCs. We propose that vocal fold fibroblasts are MSCs resident in the vocal fold lamina propria. Laryngoscope, 2010

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