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Expression of leptin receptor in nasal polyps: Leptin as a mucosecretagogue

Authors

  • Si-Youn Song MD,

    1. Department of Otorhinolaryngology–Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, South Korea
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  • Hyun-Jae Woo MD,

    1. Department of Otorhinolaryngology–Head and Neck Surgery, Gumi CHA Hospital, CHA University, Gumi, South Korea
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  • Chang Hoon Bae MD,

    1. Department of Otorhinolaryngology–Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, South Korea
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  • Yong-Woon Kim MD,

    1. Department of Physiology, College of Medicine, Yeungnam University, Daegu, South Korea
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  • Yong-Dae Kim MD

    Corresponding author
    1. Department of Otorhinolaryngology–Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, South Korea
    • Department of Otorhinolaryngology–Head and Neck Surgery, College of Medicine, Yeungnam University, 317–1, Daemyung 5-Dong, Nam-Gu, Daegu, 705-717, South Korea
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  • This research was supported by Yeungnam University research grants in 2008. The authors have no other funding, financial relationships, or conflicts of interest to disclose.

Abstract

Objectives/Hypothesis:

Leptin is a pleiotropic hormone that regulates food intake and metabolic and endocrine functions. Serum leptin levels have been reported to be increased in patients with allergic rhinitis and nasal polyposis; however, the explanation for this is unclear. We aimed to demonstrate the differential expression of leptin receptors in normal human nasal mucosa and nasal polyps, and to elucidate the effects of leptin on mucin gene expression in human nasal polyp epithelial cells.

Study Design:

Case-control and in vitro study.

Methods:

Normal ethmoid sinus mucosa was obtained from 10 subjects and used as a control; nasal polyps were obtained from 10 patients. Leptin receptor expression was analyzed using reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis. Leptin-induced expression of major respiratory mucins (MUC5AC and MUC5B) in the human nasal polyp epithelial cells was determined using RT-PCR and enzyme-linked immunosorbent assay.

Results:

The leptin receptor expression was stronger in the nasal polyps than in the normal nasal mucosa. In human nasal polyp epithelial cells, leptin increased the expression of MUC5AC and MUC5B, in a dose- and time-dependent manner, at the gene and protein levels. Leptin-induced mucin expression was inhibited by the leptin receptor antagonist.

Conclusions:

The increased expression of leptin receptors in nasal polyps implies leptin has a certain role in nasal polyposis. In addition, leptin appears to induce the expression of MUC5AC and MUC5B through leptin receptors in the human nasal polyp epithelial cells. Laryngoscope, 2010

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