The study was supported by The Swedish Larynx Foundation (Laryngfonden), The County Council of Jamtland Sweden Foundation (FoU Jämtlands läns Landsting). The authors have no other funding, financial relationships, or conflicts of interest to disclose.
Injection of human mesenchymal stem cells improves healing of scarred vocal folds: Analysis using a xenograft model†
Article first published online: 21 JUN 2010
Copyright © 2010 The American Laryngological, Rhinological, and Otological Society, Inc.
Volume 120, Issue 7, pages 1370–1375, July 2010
How to Cite
Svensson, B., Nagubothu, R. S., Cedervall, J., Le Blanc, K., Ährlund-Richter, L., Tolf, A. and Hertegård, S. (2010), Injection of human mesenchymal stem cells improves healing of scarred vocal folds: Analysis using a xenograft model. The Laryngoscope, 120: 1370–1375. doi: 10.1002/lary.20926
- Issue published online: 25 JUN 2010
- Article first published online: 21 JUN 2010
- Manuscript Accepted: 26 JAN 2010
- Vocal fold;
- mesenchymal stem cells;
- Level of Evidence: 5
The aims were to analyze if improved histological and viscoelastic properties seen after injection of human mesenchymal stem cells (hMSCs) in scarred vocal folds (VFs) of rabbits are sustainable and if the injected hMSCs survive 3 months in the VFs.
Experimental xenograft model.
Eighteen VFs of 11 New Zealand white rabbits were scarred by a bilateral localized resection. After 3 months the animals were sacrificed. Twelve VFs were dissected and stained for histology, lamina propria thickness, and relative collagen type I analyses. The hMSCs survival was analyzed using a human DNA-specific reference probe, that is, fluorescence in situ hybridization staining. Viscoelasticity, measured as the dynamic viscosity and elastic modulus, was analyzed in a parallel-plate rheometer for 10 VFs.
The dynamic viscosity and elastic modulus of hMSC-treated VFs were similar to that of normal controls and significantly improved compared to untreated controls (P < .05). A reduction in lamina propria thickness and relative collagen type 1 content were also shown for the hMSC-treated VFs compared to the untreated VFs (P < .05). The histological pictures corresponded well to the viscoelastic results. No hMSCs survived.
Human mesenchymal stem cells injected into a scarred vocal fold of rabbit enhance healing of the vocal fold with reduced lamina propria thickness and collagen type I content and restore the viscoelastic function. Laryngoscope, 2010