Characteristics of chloride transport in nasal mucosa from patients with primary ciliary dyskinesia

Authors

  • Do-Yeon Cho MD,

    1. Division of Rhinology, Department of Otolaryngology–Head and Neck Surgery, Stanford University School of Medicine, Stanford, California, U.S.A.
    2. Children's Hospital Oakland Research Institute, Oakland, California, U.S.A.
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  • Peter H. Hwang MD,

    1. Division of Rhinology, Department of Otolaryngology–Head and Neck Surgery, Stanford University School of Medicine, Stanford, California, U.S.A.
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  • Beate Illek PhD

    Corresponding author
    1. Children's Hospital Oakland Research Institute, Oakland, California, U.S.A.
    • Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr. Way, Oakland, CA 94609
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  • The authors have no other funding, financial relationships, or conflicts of interest to disclose.

  • The study protocol has been approved by Stanford University and Children's Hospital Oakland Research Institute institutional review boards.

Abstract

Objectives/Hypothesis:

Primary ciliary dyskinesia (PCD) is an inherited disorder that produces lifelong difficulties with chronic airway inflammation. Little is known about the role of chronic airway inflammation on chloride ion transport properties in PCD. This study assessed the cyclic adenosine monophosphate (cAMP)-regulated chloride (Cl) ion transport properties of freshly excised nasal mucosa from PCD compared with normal and chronic rhinosinusitis (CRS).

Study Design:

Electrophysiology study utilizing Ussing type hemi-chamber technique with three different types of nasal tissue (normal, CRS, PCD) obtained from patients during endoscopic surgery at a tertiary referral center.

Methods:

Nasal tissues were examined under short-circuit conditions, and gradient-driven Cl currents were continuously recorded. The cAMP elevating agonist (forskolin) was added to stimulate cystic fibrosis transmembrane conductance regulator-mediated Cl secretion. To prevent misinterpretation of flux measurement, Cl transport inhibitors were used at the end of all experiments. Basal Cl currents (ICl) and changes in ICl to forskolin (ΔICl) were compared between normal, CRS, and PCD nasal tissues.

Results:

Forskolin stimulated Cl currents across all different types of nasal epithelia. The Cl secretory response was effectively blocked by the Cl ion transport inhibitors. ICl were significantly higher in normals (155.0 ± 9.3 μA/cm2) compared to CRS (79.1 ± 15.0 μA/cm2) and PCD (70.9 ± 20.4 μA/cm2) (P = .005). ΔICl in CRS (14.8 ± 2.3 μA/cm2) and PCD (12.2 ± 2.4 μA/cm2) were markedly diminished compared to normals (28.3 ± 4.7 μA/cm2) (P = .024).

Conclusions:

PCD tissues were characterized by impaired ICl and ΔICl. Both parameters were reduced by 54.3% and 56.9% in PCD when compared to normals. Laryngoscope, 2010

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