Accepted for oral presentation at the 131st Annual Meeting of The American Laryngological Association, Las Vegas, Nevada, April 28–29, 2010.
Version of Record online: 18 JUN 2010
Copyright © 2010 The American Laryngological, Rhinological, and Otological Society, Inc.
Volume 120, Issue 7, pages 1354–1362, July 2010
How to Cite
Kobler, J. B., Chang, E. W., Zeitels, S. M. and Yun, S.-H. (2010), Dynamic imaging of vocal fold oscillation with four-dimensional optical coherence tomography. The Laryngoscope, 120: 1354–1362. doi: 10.1002/lary.20938
This work was supported in part by the Eugene B. Casey Foundations and the Institute of Laryngology and Voice Restoration, NIH grant RC1DK086242, and a Wellman graduate student fellowship (Ernest W. Chang). The authors have no other funding, financial relationships, or conflicts of interest to disclose.
- Issue online: 25 JUN 2010
- Version of Record online: 18 JUN 2010
- Manuscript Accepted: 25 FEB 2010
- Optical coherence tomography;
- vocal fold;
- mucosal wave;
Optical coherence tomography (OCT) can provide high-resolution (∼10–15 μm/pixel) images of vocal fold microanatomy, as demonstrated previously. We explored physiologically triggered Fourier-domain OCT for imaging vocal folds during phonation. The goal is to visualize dynamic histological cross sections and four-dimensional data sets where multiple planes are displayed in synchronized motion. If feasible, this approach could be a useful research tool and spur development of new clinical instrumentation.
A Fourier-domain, triggered OCT system was created and tested in experiments on excised calf larynges to obtain preliminary observations and characterize important factors affecting image quality.
Larynges were imaged during phonation driven by warm, humidified air. A subglottal pressure signal was used to synchronize the OCT system with the phonatory cycle. Image sequences were recorded as functions of anatomical location or subglottal pressure. Implant materials were also imaged during vibration, both in isolation and after injection into a vocal fold.
Oscillations of epithelium and lamina propria were observed, and parameters such as shape, amplitude, and velocity of the vocal fold mucosal waves were found to be measurable. Ripples of mucosal wave as small as 100 μm in vertical height were clearly visible. Internal strain was also observed in normal and implanted vocal folds.
Four-dimensional OCT of the vocal fold may help to more directly relate biomechanics to anatomy and disease. It may also be useful for assaying the functional rheology of implants in the context of real tissue. With further development, this technology has potential for clinical endoscopic application. Laryngoscope, 2010