Portions of this manuscript were presented at the 2010 American Laryngological Association Combined Otolaryngology Spring Meetings, Las Vegas, Nevada, U.S.A., April 28–May 2, 2010.
Version of Record online: 17 AUG 2010
Copyright © 2010 The American Laryngological, Rhinological, and Otological Society, Inc.
Volume 120, Issue 9, pages 1826–1831, September 2010
How to Cite
Branski, R. C., Zhou, H., Sandulache, V. C., Chen, J., Felsen, D. and Kraus, D. H. (2010), Cyclooxygenase-2 signaling in vocal fold fibroblasts. The Laryngoscope, 120: 1826–1831. doi: 10.1002/lary.21017
This work was funded by the NIH/NIDCD (RO3 DC010267), Hackers for Hope, The Langeloth Foundation, and the Garban Fund. The authors have no other funding, financial relationships, or conflicts of interest to disclose.
- Issue online: 23 AUG 2010
- Version of Record online: 17 AUG 2010
- Manuscript Accepted: 13 APR 2010
- Manuscript Revised: 26 MAR 2010
- Manuscript Received: 8 FEB 2010
- Vocal fold;
Inflammation and its role in a coordinated fibroplastic response, which disrupts the structure of the vocal folds following injury, is critical. Cyclooxygenase-2 (COX-2) is an important enzyme involved in both inflammation and fibrosis; in addition, it is a prime target for therapeutic intervention. We sought to study this pathway in vocal fold fibroblasts to provide a foundation for future interventional studies.
Human vocal fold fibroblasts were incubated with IL-1β to determine the effects on COX-2 signaling, along with upstream regulatory mechanisms and downstream mediators of wound healing. In vitro methods to assess mRNA expression, as well as intracellular and secreted protein (sodium dodecyl sulfate polyacrylamide gel electrophoresis and enzyme-linked immunosorbent assay) were employed.
IL-1β regulation of COX-2 mRNA and protein levels was dose and time dependent and IL-1β altered PGE2 metabolism, via regulation of both synthetic and degradative enzymes. IL-1β increased nuclear factor (NF)-κB activation and nuclear translocation. Inhibition of the p50 and p65 subunits of NF-κB decreased IL-1β-induced COX-2 transcription. IL-1β also altered mRNA expression of four cell-surface prostaglandin receptors.
Inflammation and fibrosis are important in the vocal fold pathophysiologic response to injury. Our data suggest that COX-2 and PGE2 are inducible in human vocal fold fibroblasts, and this response appears to be NF-κB-dependent. We purport this fundamental investigation will lead to increased insight regarding injury and repair in the vocal folds, with the ultimate goal of developing novel clinical care paradigms. Laryngoscope, 2010