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Comparison of gentamicin distribution in the inner ear following administration via the endolymphatic sac or round window

Authors

  • Yiliang Zhang MD,

    1. Department of Thoracic Surgery, Fudan University Shanghai Cancer Center and the Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China
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  • Ru Zhang MD, PhD,

    1. Department of Otology & Skull Base Surgery , Eye Ear Nose & Throat Hospital, Fudan University, Shanghai, People's Republic of China
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  • Chunfu Dai MD, PhD,

    Corresponding author
    1. Department of Otology & Skull Base Surgery , Eye Ear Nose & Throat Hospital, Fudan University, Shanghai, People's Republic of China
    2. Institutes of Brain Science , Fudan University, Shanghai, People's Republic of China
    • Department of Otology & Skull Base Surgery, Eye Ear Nose & Throat Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, People's Republic of China
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  • Peter S. Steyger PhD,

    1. Oregon Hearing Research Center , Oregon Health & Sciences University, Portland, Oregon, U.S.A.
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  • Yongfu Yu MD

    1. Department of Biostatistics , School of Public Health, Fudan University, People's Republic of China
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  • The authors declare that there are no conflicts of interest.

Abstract

Objective/Hypothesis:

The distribution of gentamicin in the inner ear via the endolymphatic sac (ES) or round window (RW) routes was investigated.

Study Design:

Experimental study.

Methods:

A fluorescent gentamicin-Texas Red conjugate (GTTR) was adopted to visualize the gentamicin. Adult guinea pigs were treated with GTTR applied to the ES or RW, the animals were allowed to recover for 3 days, then confocal microscopy was used to observe and quantify GTTR distributions in cochlear and vestibular sensory epithelium.

Results:

When GTTR was applied via the ES, strong GTTR labeling was observed in the vestibule while little GTTR was detected in the cochlea (P < .0001). However, distinct GTTR fluorescence was observed in the cochlea and vestibule following RW application (P = .7967). There was less GTTR labeling in cochlea via ES application than through RW administration (P < .0001).

Conclusions:

ES drug application may be preferable for the treatment of intractable Meniere's disease. Laryngoscope, 2010

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