This work was supported by National Taiwan University Hospital, Taipei, Taiwan, grant number: NTUH97-M-1009. The authors have no other funding, financial relationships, or conflicts of interest to disclose.
Evaluation of guinea pig model for ocular and cervical vestibular-evoked myogenic potentials for vestibular function test†
Article first published online: 17 AUG 2010
Copyright © 2010 The American Laryngological, Rhinological, and Otological Society, Inc.
Volume 120, Issue 9, pages 1910–1917, September 2010
How to Cite
Yang, T.-H., Liu, S.-H. and Young, Y.-H. (2010), Evaluation of guinea pig model for ocular and cervical vestibular-evoked myogenic potentials for vestibular function test. The Laryngoscope, 120: 1910–1917. doi: 10.1002/lary.21056
- Issue published online: 23 AUG 2010
- Article first published online: 17 AUG 2010
- Manuscript Accepted: 21 MAY 2010
- Manuscript Revised: 16 MAY 2010
- Manuscript Received: 21 MAR 2010
- Air-conducted sound;
- bone-conducted vibration;
- cervical vestibular-evoked myogenic potential;
- ocular vestibular-evoked myogenic potential;
- guinea pigs;
- Level of Evidence: 2c.
This study used air-conducted sound (ACS) and bone-conducted vibration (BCV) stimuli in eliciting ocular vestibular-evoked myogenic potential (oVEMP) and cervical VEMP (cVEMP) in guinea pigs.
Ten guinea pigs were treated with gentamicin (4 mg) on the left ear, whereas the right ear served as a control. One week after treatment, each animal underwent oVEMP and cVEMP tests using ACS and BCV modes in a randomized order, and was sacrificed for morphological study.
Using ACS mode, oVEMPs were absent in all 10 (100%) animals despite the stimulus intensity increased up to 120 dB pe SPL. Conversely, using BCV mode, oVEMPs were present on the left (lesion) eye, and absent on the right (control) eye in all (100%) animals. For the cVEMPs via ACS mode, all right (control) necks had clear cVEMPs, and all (100%) left (lesion) necks revealed absent cVEMPs. However, via BCV mode, all right (control) necks and six (60%) left (lesion) necks showed clear cVEMPs. Morphological study demonstrated substantial loss of hair cells in the utricular and saccular macula.
The cVEMP test via ACS mode is specific for investigating the saccular disorder, whereas the oVEMP test via BCV mode is preferable for investigating the utricular disorders in humans. The guinea pig model is consistent with the findings of humans. Restated, appropriate animal models for cVEMP and oVEMP in guinea pigs are via ACS and BCV modes, respectively. Laryngoscope, 2010