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Gentamicin alters nitric oxide production in semicircular canals and otolith organs

Authors

  • Kai Helling MD,

    Corresponding author
    1. Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany
    • Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstrasse 1, D-55131 Mainz, Germany
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  • Ulf-Rüdiger Heinrich PhD,

    1. Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany
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  • Lukas Heusgen Cand. Med,

    1. Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany
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  • Jürgen Brieger PhD,

    1. Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany
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  • Irene Schmidtmann Dipl. Math,

    1. Medical Statistics, Epidemiology and Informatics, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany
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  • Huige Li MD, PhD,

    1. Department of Pharmacology, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany
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  • Wolf J. Mann MD, PhD

    1. Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany
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  • This work was supported by a grant from the Preventive Medicine Project of the University Medical Center, Johannes Gutenberg-University Mainz. This manuscript is part of a candidate thesis presented by Lukas Heusgen at the University Medical Center of the Johannes Gutenberg-University Mainz. The authors have no other funding, financial relationships, or conflicts of interest to disclose.

Abstract

Objectives/Hypothesis:

Intratympanic application of gentamicin is an important therapeutic option to control vertigo spells in Ménière's disease. Low doses eliminate the function of semicircular canal ampullae (SCCA) and saccule in most patients, although utricular function is maintained in many cases. Local alteration in free radical production might be responsible for these differences. Therefore, the gentamicin-induced nitric oxide (NO) production was determined in an animal model using separate organ cultures of SCCA, saccule, and utricle.

Study Design:

Prospective pilot study in male guinea pigs.

Methods:

SCCA, saccule, and utricle of 28 guinea pigs were isolated and incubated separately for 6 hours in cell culture medium. Gentamicin was administered in two different concentrations (0.4 mg/mL and 0.8 mg/mL) to organ cultures of 16 animals. Tissues from 12 animals were used as controls. Nitric oxide was quantified by chemiluminescence.

Results:

Gentamicin led to an NO increase of about 70% in the saccule, an NO reduction of more than 70% in SCCA, and an NO reduction of 36% in the utricle.

Conclusions:

The selective effects of gentamicin on the NO production in the different sensory areas of the vestibular organ have to be taken into account in the therapy of Ménière's disease. Laryngoscope, 2010

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