The authors have no financial disclosures for this article.
Article first published online: 13 JAN 2011
Copyright © 2010 The American Laryngological, Rhinological, and Otological Society, Inc.
Volume 121, Issue 2, pages 361–364, February 2011
How to Cite
Hwang, J.-H., Chen, J.-C., Yang, S.-Y., Wang, M.-F., Liu, T.-C. and Chan, Y.-C. (2011), Expression of COX-2 and NMDA receptor genes at the cochlea and midbrain in salicylate-induced tinnitus. The Laryngoscope, 121: 361–364. doi: 10.1002/lary.21283
The authors have no onflicts of interest to disclose.
This study was supported by grants from the National Science Council, Taiwan (NSC-95-231693-B-126-008) and the Taichung Veterans General Hospital and Providence University (TCVGH-PU988104) Taichung, Taiwan.
- Issue published online: 26 JAN 2011
- Article first published online: 13 JAN 2011
- Manuscript Accepted: 28 JUL 2010
- Manuscript Received: 15 APR 2010
- National Science Council
- Taichung Veterans General Hospital
- COX-2 gene;
- NMDA receptor gene;
- Level of Evidence: 5
The expression of the genes for cyclooxygenase (COX) and NMDA receptor (NR) has seldom been reported in tinnitus. We hypothesized that expression of COX-2 and NR was altered in the cochlea and midbrain in salicylate-induced tinnitus.
Experimental study on mice.
We evaluated the tinnitus score and mRNA expression levels of COX-2 and NR subtype 2B (NR2B) in the cochlea and midbrain in response to intraperitoneal injections of salicylate for 4 days.
At day 4 of tinnitus induction, the mean weights of the whole body and midbrain did not change greatly in both control and salicylate groups. The tinnitus score was not elevated from day 1 to day 4 in the control group, but increased day by day in the salicylate group. The mRNA expression level of COX-2 decreased slightly in the salicylate group in the cochlea (1.1 ± 0.33 vs. 1.3 ± 0.49, P = .0752) and in the midbrain (0.9 ± 0.10 versus 1.0 ± 0.35, P = .0489). Inversely, the expression levels of the NR2B gene increased moderately in the salicylate group in the cochlea (3.7 ± 0.47 versus 2.3 ± 1.13, P < 0.0001) and in the midbrain (1.6 ± 0.64 versus 1.0 ± 0.44, P = .0007).
Salicylate induced tinnitus and altered the expression of the COX-2 and NR2B genes in the cochlea and midbrain of mice. These findings might contribute to further understanding of pathophysiology and therapy of tinnitus. Laryngoscope, 2011