Experiments on excised larynges were completed per regulations at Purdue University.
Article first published online: 6 SEP 2011
Copyright © 2011 The American Laryngological, Rhinological, and Otological Society, Inc.
Volume 121, Issue 10, pages 2180–2184, October 2011
How to Cite
Alper, R., Fu, X., Erickson-Levendoski, E., Zheng, W. and Sivasankar, M. (2011), Acute stress to excised vocal fold epithelium from reactive oxygen species. The Laryngoscope, 121: 2180–2184. doi: 10.1002/lary.22157
Presented at the annual meeting of the American Laryngological Association, Chicago, Illinois, U.S.A., April 27–28, 2011.
Funded by the National Institute on Deafness and other Communication Disorders (NIH), Grant #008690, and National Institute of Environmental Health Sciences (NIH), Grant #008146. The authors have no other funding, financial relationships, or conflicts of interest to disclose.
Editor's Note: This Manuscript was accepted for publication May 20, 2011
- Issue published online: 21 SEP 2011
- Article first published online: 6 SEP 2011
- Manuscript Accepted: 20 MAY 2011
- Manuscript Revised: 13 MAY 2011
- Manuscript Received: 1 MAR 2011
- vocal fold epithelia;
- reactive oxygen species
Vocal fold epithelium is exposed to reactive oxygen species from the inhaled environment and from tissue inflammation. The objective of this study was to explore the functional and structural consequences of reactive oxygen species exposure on vocal fold epithelium.
In vitro, prospective study design.
Hydrogen peroxide (H2O2), a common reactive oxygen species, was utilized in this study. Freshly excised, viable porcine vocal fold epithelia (N = 32) were exposed to H2O2 or sham challenge for 2 hours. Electrophysiology, western blotting, and light microscopy were used to quantify the functional and structural effects of reactive oxygen species on vocal fold epithelia.
Exposure to reactive oxygen species did not significantly alter transepithelial resistance. There was a small, nonsignificant trend for decreased concentration of epithelial junctional complex protein with reactive oxygen species challenge. Minimal changes to the gross structural appearance of vocal fold epithelia were also noted.
The stratified squamous epithelia of the vocal folds effectively defend against an acute reactive oxygen species challenge. The current study lays the groundwork for future investigations on the effects of reactive oxygen species on vocal fold epithelia that are compromised from phonotrauma.