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Bioengineered prosthesis with allogenic heterotopic fibroblasts for cricoid regeneration


  • Delivered as an oral presentation at the American Laryngological Association Annual Meeting, Chicago, Illinois, U.S.A., April 27–28, 2011.

  • This work was supported by Grant-in-Aid for Scientific Research (KAKENHI), 2010–2011, with Mika Nomoto as Principal Investigator and by Fukushima Medical University. The authors have no other funding, financial relationships, or conflicts of interest to disclose.



This study aimed to determine the effectiveness of a bioengineered prosthesis with allogenic heterotopic fibroblasts for cricoid regeneration.

Study Design:

Experimental study using a rat model.


Bioengineered prosthetic grafts consisting of collagen sponge, polypropylene mesh, collagen gel, and allogenic dermal fibroblasts were implanted into rat cricoid defects. Control grafts were composed of collagen sponge and polypropylene mesh. Regenerated subglottic tissue was subsequently examined histologically.


Epithelization and changes of collagen fibers occurred more rapidly in the bioengineered model than in the control model. Neogenetic cartilage was observed to form around the ends of cricoid cartilage in both models.


Heterotopic fibroblasts appear to accelerate epithelial and subepithelial regeneration in a rat model. Laryngoscope, 2012