Research supported by NIH grants R03 DC 008400 and R01 DC 011338 from the National Institute of Deafness and other Communication Disorders. The authors have no other funding, financial relationships, or conflicts of interest to disclose.
Feasibility and acute healing of vocal fold microflap incisions in a rabbit model†
Article first published online: 17 JAN 2012
Copyright © 2011 The American Laryngological, Rhinological, and Otological Society, Inc.
Volume 122, Issue 3, pages 600–605, March 2012
How to Cite
Suehiro, A., Bock, J. M., Hall, J. E., Garrett, C. G. and Rousseau, B. (2012), Feasibility and acute healing of vocal fold microflap incisions in a rabbit model. The Laryngoscope, 122: 600–605. doi: 10.1002/lary.22470
- Issue published online: 21 FEB 2012
- Article first published online: 17 JAN 2012
- Manuscript Accepted: 28 OCT 2011
- Manuscript Revised: 24 OCT 2011
- Manuscript Received: 27 SEP 2011
- vocal fold;
- wound healing;
- gene expression;
- Level of Evidence: 3b. Individual case-control study
The purpose of this study was to investigate the feasibility of performing mucosal elevation of a vocal fold microflap in a rabbit model and to measure the acute healing of rabbit microflap incisions compared to control vocal folds.
Prospective animal study.
Ten New Zealand white rabbits were used in this study. All rabbits received a 3-mm incision through the epithelium of one vocal fold using a sickle knife and mucosal elevation through this incision using a microlaryngeal fine-angled spatula. The contralateral vocal fold was left intact to serve as an internal control. Student t tests were used to investigate differences in epithelial thickness, immunohistochemical staining of CD45, and inflammatory and profibrotic gene expression between vocal folds undergoing microflap and control.
Exposure of the rabbit larynx was achieved, allowing for the identification of a surgical plane and the creation of a microflap and elevation of the vocal fold mucosa. Hematoxylin-and-eosin staining revealed no significant differences in epithelial thickness, immunohistochemistry for CD45 showed no significant differences in CD45-positive cells, and quantitative polymerase chain reaction revealed no significant differences in interleukin-1β, transforming growth factor β-1, or cyclooxygenase-2 gene expression between vocal folds undergoing microflap and control.
We demonstrate the feasibility of vocal fold microflap surgery in a rabbit model. With the advantage of greater access to primers and antibodies for molecular biologic studies, the application of the microflap technique in a small-animal model such as rabbit has broad implications for future experimental investigations in laryngology.