Get access

Nimodipine accelerates reinnervation of denervated rat thyroarytenoid muscle following nerve-muscle pedicle implantation

Authors

  • Kohei Nishimoto MD,

    Corresponding author
    1. Department of Otolaryngology Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, Kumamoto, Japan
    • Department of Otolaryngology Head and Neck Surgery, Kumamoto University Graduate School of Medicine, 1-1-1 Honjo, Kumamoto 860-8556, Japan
    Search for more papers by this author
  • Yoshihiko Kumai MD PhD,

    1. Department of Otolaryngology Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, Kumamoto, Japan
    Search for more papers by this author
  • Ryosei Minoda MD, PhD,

    1. Department of Otolaryngology Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, Kumamoto, Japan
    Search for more papers by this author
  • Eiji Yumoto MD, PhD

    1. Department of Otolaryngology Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, Kumamoto, Japan
    Search for more papers by this author

  • This work was supported in part by a scholarship for the Graduate School of Medical Sciences, Kumamoto University, Japan. The authors have no other funding, financial relationships, or conflicts of interest to disclose.

Abstract

Objectives/Hypothesis:

To determine whether nimodipine, an L-type voltage-operated calcium channel antagonist that is an accelerator of axonal regeneration following peripheral nerve injury, can expedite reinnervation of denervated rat thyroarytenoid (TA) muscle following nerve-muscle pedicle (NMP) flap implantation.

Study Design:

A quantitative histologic and physiologic assessment of the TA muscle following NMP flap implantation, with or without nimodipine treatment.

Methods:

Using 72 Wistar rats, we performed a transection on the left recurrent laryngeal nerve, followed by NMP flap implantation. Thirty-six animals received nimodipine treatment (NIMO [+] group), and the remaining 36 animals received no nimodipine treatment (NIMO [−] group). As a control, 18 animals were subjected only to transection of the left recurrent laryngeal nerve. We performed a histologic assessment for muscle area, axon, nerve terminals (NTs), and acetylcholine receptors (AchRs) in the TA muscle and electromyography at 2, 4, and 10 weeks after surgery.

Results:

Muscle area, ratio of the number of NTs to that of AchRs (NT/AchR ratio), and evoked action potential in the TA muscle were significantly greater in the NIMO (+) group than in the NIMO (−) group (P < .05) at 4 weeks. At 10 weeks, the NT/AchR ratio was significantly greater in the NIMO (+) group than in the NIMO (−) group (P < .05).

Conclusions:

We found that nimodipine accelerated reinnervation of the denervated TA muscle following NMP flap implantation.

Ancillary