Cystic fibrosis transmembrane conductance regulator modulation by the tobacco smoke toxin acrolein§

Authors


  • Presented at the American Academy of Otolaryngology–Head and Neck Surgery Annual Meeting, San Francisco, California, U.S.A., September 11–14, 2011.

  • This research was funded by the National Institutes of Health/National Heart, Lung, and Blood Institute (1K08HL107142-01) and Flight Attendant's Medical Research Institute Young Clinical Scientist Award (072218) to Bradford A. Woodworth, MD; and NIH/NIDDK (5P30DK072482-03) to Eric J. Sorscher, MD.

  • §

    Bradford A. Woodworth, MD is a consultant for ArthroCare ENT and Gyrus ENT. Eric Sorscher, MD, and Bradford Woodworth, MD, are inventors on a patent pending regarding the use of chloride secretagogues for therapy of sinus disease, no. 35 U.S.C. n111(b) and 37 C.F.R n.53 (c), in the US Patent and Trademark Office.

  • The authors have no other funding, financial relationships, or conflicts of interest to disclose.

Abstract

Objectives/Hypothesis:

Evidence indicates that decreased mucociliary clearance (MCC) is a major contributing feature to chronic rhinosinusitis. Tobacco-smoke exposure is thought to inhibit transepithelial Cl secretion, a major determinant of airway surface liquid hydration and MCC. The objective of the current study was to evaluate the effects of acrolein exposure (a prominent tobacco smoke toxin) on vectorial Cl transport through the major apical anion channel cystic fibrosis transmembrane conductance regulator (CFTR) in sinonasal epithelium.

Study Design:

In vitro investigation.

Methods:

Primary murine nasal septal epithelia (MNSE; wild-type and transgenic CFTR−/−) cultures were exposed to acrolein in Ussing chambers and the effects on Cl secretion investigated using pharmacologic manipulation. Cellular cyclic adenosine monophosphate (cAMP) signaling and cytotoxicity were also investigated.

Results:

Acrolein stimulated Cl secretion (ΔISC—change in short-circuit current in μA/cm2) at concentrations similar to smoker's airways (100 μM, 15.8 ± 2.2 vs. 2.4 ± 0.8 [control]; P < .0001), suppressed forskolin-stimulated C− transport at 300 μM (13.3 ± 1.2 vs. 19.9 ± 1.0; P < .01), and completely abolished all transport at 500 μM (−1.1 ± 1.6). Stimulated Cl secretion was solely reliant upon the presence of CFTR (confirmed in transgenic CFTR−/− MNSE), but independent of cAMP signaling. Inhibition at higher concentrations was not secondary to cellular cytotoxicity.

Conclusions:

The present study demonstrates that acrolein has complex but pronounced interaction with the major apical Cl transport mechanism that uses CFTR. Further investigations are required to determine acrolein's impact as a tobacco smoke constituent on mucociliary transport.

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