Get access

Low SPINK5 expression in chronic rhinosinusitis

Authors

  • Kai Fruth MD,

    Corresponding author
    1. Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center, Johannes Gutenberg University Mainz, Mainz, Germany
    • Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University Mainz, Langenbeckstrasse 1, 55101 Mainz, Germany
    Search for more papers by this author
  • Gyula Goebel MD,

    1. Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center, Johannes Gutenberg University Mainz, Mainz, Germany
    2. Institute of Medical Biostatistics, Epidemiology, and Informatics, University Medical Center, Johannes Gutenberg University Mainz, Mainz, Germany
    Search for more papers by this author
  • Dimitrios Koutsimpelas MD,

    1. Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center, Johannes Gutenberg University Mainz, Mainz, Germany
    Search for more papers by this author
  • Jan Gosepath MD, PhD,

    1. Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center, Johannes Gutenberg University Mainz, Mainz, Germany
    Search for more papers by this author
  • Irene Schmidtmann MSc,

    1. Institute of Medical Biostatistics, Epidemiology, and Informatics, University Medical Center, Johannes Gutenberg University Mainz, Mainz, Germany
    Search for more papers by this author
  • Wolf J. Mann MD, PhD,

    1. Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center, Johannes Gutenberg University Mainz, Mainz, Germany
    Search for more papers by this author
  • Juergen Brieger PhD

    1. Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center, Johannes Gutenberg University Mainz, Mainz, Germany
    Search for more papers by this author

  • This study was approved by the institutional review board and performed in accordance to the actual version of the declaration of Helsinki.

  • The authors have no funding, financial relationships, or conflicts of interest to disclose.

Abstract

Objectives/Hypothesis:

Chronic rhinosinusitis (CRS) is a multifactorial disease that probably arises as a result of genetic diversity and environmental factors. SPINK5 is a serine protease inhibitor, which is supposed to be an important regulator of epithelial barrier maintenance. The role of SPINK5 polymorphisms and expression in CRS, especially in individuals with aspirin intolerance, is unclear.

Study Design:

SPINK5 single-nucleotide polymorphisms (SNPs) and SPINK5 expression levels were correlated with CRS without (CRSsNP) and with nasal polyps (CRSwNP), aspirin intolerance, asthma, and allergies.

Methods:

One hundred four nasal tissue samples, 15 from patients with CRSsNP, 59 from patients with CRSwNP, and 30 from healthy controls of the inferior turbinate, were analyzed for their SPINK5 status. Genotypes of four SPINK5 single nucleotide polymorphism (SNPs; G1258A, G2475T, A2915G, and A1103G), as well as SPINK5 mRNA expression levels, were determined by polymerase chain reaction.

Results:

No correlation between any SPINK5 SNP and CRSsNP, CRSwNP, or allergies and asthma was observed. The heterozygous SNPs G1258A and A1103G were observed more frequently in aspirin-intolerant patients; the homozygous (A/A) genotype of SNP 1258 and the homozygous (G/G) genotype SNP 1103 were less frequent. There was no correlation between the analyzed SNPs and the level of SPINK5 expression. It was noted that in individuals with CRSwNP, aspirin intolerance, and allergies, SPINK5 expression was lowered.

Conclusions:

G1258A and A1103G polymorphisms are distinctive for the aspirin intolerance syndrome. Lowered SPINK5 expression might be a contributing factor leading to CRS, and appears to be characteristic for patients suffering from aspirin intolerance and from allergies. Laryngoscope, 2012

Get access to the full text of this article

Ancillary