The authors have other no funding, financial relationships, or conflicts of interest to disclose.
Expression levels of endogenous hydrogen sulfide are altered in patients with allergic rhinitis†
Article first published online: 9 JAN 2013
Copyright © 2013 The American Laryngological, Rhinological, and Otological Society, Inc.
Volume 123, Issue 3, pages 557–563, March 2013
How to Cite
Park, S. J., Kim, T. H., Lee, S. H., Ryu, H. Y., Hong, K. H., Jung, J. Y., Hwang, G. H. and Lee, S. H. (2013), Expression levels of endogenous hydrogen sulfide are altered in patients with allergic rhinitis. The Laryngoscope, 123: 557–563. doi: 10.1002/lary.23466
- Issue published online: 26 FEB 2013
- Article first published online: 9 JAN 2013
- Manuscript Accepted: 4 MAY 2012
- Manuscript Revised: 4 APR 2012
- Manuscript Received: 1 MAR 2012
- Grant-in-Aid for Scientific Research from the Communication Disorders Center, Korea University, Korea
- National Research Foundation of Korea
- Ministry of Education, Science, and Technology. Grant Number: 2010-0004094
- Korea University grant, Korea
- Cystathione γ-lyase;
- cystathione β-synthase;
- hydrogen sulfide;
- mild persistent allergic rhinitis;
- moderate/severe persistent allergic rhinitis;
- Level of Evidence: 2c
Hydrogen sulfide (H2S), the third endogenous gaseous transmitter, may be a crucial mediator in airway hyper-responsiveness and airway inflammation, including asthma. To elucidate the role of H2S in allergic rhinitis, the present study was undertaken to determine the level of expression of H2S in healthy nasal mucosa and mild and moderate/severe persistent allergic nasal mucosa as well as peripheral blood obtained from each patient. The expression and distribution pattern of the H2S-synthesizing enzymes cystathione γ-lyase (CSE) and cystathione β-synthase (CBS) were investigated in healthy and allergic nasal mucosa.
Controlled, prospective study.
The concentration of H2S in nasal mucosa and plasma was determined by zinc trap spectrophotometry. The expression levels and patterns of distribution of CSE and CBS mRNA and proteins were evaluated using real time polymerase chain reaction, Western blot, and immunohistochemistry.
The levels of expression of H2S in nasal mucosa and plasma were increased in patients with mild and moderate/severe persistent allergic rhinitis compared with healthy controls. CSE was localized in vascular endothelium and surrounding muscles, and submucosal glands, whereas CBS was exclusively distributed in the superficial epithelium and submucosal glands. Their expression levels were increased in mild and moderate/severe persistent allergic rhinitis.
The current findings indicate that, in parallel with increased expression levels of CSE and CBS, H2S is upregulated in nasal mucosa and plasma of allergic patients. Based on localization of CSE and CBS, H2S may play multiple functions in human nasal mucosa, contributing to the development of allergic symptoms such as rhinorrhea, sneezing, and nasal stuffiness. Laryngoscope, 2013