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Role of circulating MSCs in vocal fold wound healing

Authors

  • Satoshi Ohno MD, PhD,

    1. Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
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  • Shigeru Hirano MD, PhD,

    Corresponding author
    1. Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    • Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606–8507, Japan
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  • Shin-ichi Kanemaru MD, PhD,

    1. Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    2. Department of Otolaryngology, Regenerative Treatment for Tympanic Membrane, The Foundation for Biomedical Research and Innovation, Kobe, Japan
    3. Department of Otolaryngology, Head and Neck Surgery, Medical Research Institute, Kitano Hospital, Osaka, Japan
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  • Masanobu Mizuta MD,

    1. Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
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  • Seiji Ishikawa MD,

    1. Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
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  • Ichiro Tateya MD, PhD,

    1. Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
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  • Tatsuo Nakamura MD, PhD,

    1. Department of Bioartificial Organs, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
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  • Juichi Ito MD, PhD

    1. Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
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  • This paper was presented at the 2012 spring meeting of the American Broncho-Esophagological Association.

  • The authors have no conflicts of interest to disclose. This study was supported by MEXT KAKENHI, Mitsui Sumitomo Insurance Welfare Foundation and the National Institute of Biomedical Innovation.

Abstract

Objectives/Hypothesis:

Vocal fold injury can cause intractable scarring resulting in dysphonia. Mesenchymal stem cells (MSCs) have great therapeutic potential in wound healing. They continuously circulate in the peripheral blood and migrate into wound sites where they induce regenerative effects. However, their roles in vocal fold wound healing are poorly understood because few MSCs exist in the peripheral blood and there is no specific marker to identify them. The present study evaluates how intravenously injected MSCs affect vocal fold wound healing using Green Fluorescent Protein (GFP) -labeled MSCs.

Study Design:

Prospective study using animal model.

Methods:

GFP-labeled MSCs were obtained from femurs of GFP transgenic Sprague-Dawley rats and incubated in culture. Sprague-Dawley rats underwent intravenous injection of GFP-labeled MSCs (1.0 × 106 cells) immediately after vocal fold injury. Histological examination was performed.

Results:

Injected MSCs were distributed throughout the vocal fold wound site from day 1 up to day 56. These vocal folds showed increased hepatocyte growth factor (HGF)-positive cells within the wound and improved wound healing compared with sham-treated folds.

Conclusion:

Circulating MSCs can migrate to vocal fold wound sites and upregulate the expression of HGF during wound healing; thus, they are considered to play a significant role in wound healing within the vocal folds. Laryngoscope, 2012

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