Presented at the Triological Society Combined Sections Meeting, San Diego, California, U.S.A., April 18–22, 2012.
Head and Neck
Article first published online: 8 JAN 2013
Copyright © 2013 The American Laryngological, Rhinological, and Otological Society, Inc.
Volume 123, Issue 3, pages 641–645, March 2013
How to Cite
Godoy, J. M., Sewell, A., Johnston, B., Brown, B. T., Lu, X., Sinard, R. J., Rohde, S., Mannion, K., Netterville, J. L. and Yarbrough, W. G. (2013), Viable biobanking of primary head and neck squamous cell carcinoma. The Laryngoscope, 123: 641–645. doi: 10.1002/lary.23674
This study was supported by an endowment to the Barry Baker Laboratory for Head and Neck Oncology, Vanderbilt Ingram Cancer Center, Vanderbilt Bill Wilkerson Center, Vanderbilt Department of Otolaryngology, and Vanderbilt Clinical and Translational Science Awards grant UL1 RR024975-01 from National Center for Research Resources/National Institutes of Health.
The authors have no other funding, financial relationships, or conflicts of interest to disclose.
- Issue published online: 26 FEB 2013
- Article first published online: 8 JAN 2013
- Manuscript Accepted: 25 JUL 2012
- Manuscript Revised: 20 JUN 2012
- Manuscript Received: 21 APR 2012
- Head and neck;
- oral cavity;
- Level of Evidence: N/A
To determine the feasibility of viable storage of head and neck squamous cell carcinoma (HNSCC) for regrowth of cells in culture.
Laboratory-based translational study.
Methods for intermediate-term frozen storage of viable HNSCC were explored using small pieces of primary tumor and dissociated HNSCC cells after short-term culture. Viable cells after freezing were confirmed by adherence to tissue culture plates, cell morphology, and increased cell or colony density. Two cultures were immunostained for cytokeratin to confirm epithelial origin of viable cultured cells after freezing.
Six primary HNSCCs (two oral cavity, three larynx, one oropharynx) and two HNSCCs that had been passaged through a xenograft (two oral cavity) were dissociated to single cells and grown in short-term cell culture for 0 to 12 passages. After short-term culture, cells were frozen for up to 8 months, thawed, and replated. Frozen cells derived from all tumors (six primary and two xenografts) were successfully replated with cultures lasting >7 days with seven of eight tumors presenting increased colony or cell density over 1 week of growth after freezing. In total, 15 of 15 tested samples derived from six primary and two xenografted HNSCCs were viable after freezing.
In the current study, we show that biopreservation of primary or xenografted HNSCC using short-term cell culture is feasible. Initial short-term cell culture was required for successful storage and viability of frozen cells. These proof-of-principle studies, if more widely implemented, could improve preclinical testing of new therapies for HNSCC. Laryngoscope, 2013