Financial Funding: Department of Otolaryngology, New York Eye and Ear Infirmary. The authors have no other funding, financial relationships, or conflicts of interest to disclose.
Development of the rat larynx: A histological study
Article first published online: 5 AUG 2013
Copyright © 2013 The American Laryngological, Rhinological and Otological Society, Inc.
Volume 123, Issue 12, pages 3093–3098, December 2013
How to Cite
Alli, O., Berzofsky, C., Sharma, S. and Pitman, M. J. (2013), Development of the rat larynx: A histological study. The Laryngoscope, 123: 3093–3098. doi: 10.1002/lary.24145
- Issue published online: 25 NOV 2013
- Article first published online: 5 AUG 2013
- Manuscript Accepted: 18 MAR 2013
- Manuscript Revised: 11 FEB 2013
- Manuscript Received: 16 JUL 2012
- recurrent laryngeal nerve
To evaluate and describe the cartilaginous and muscular development of the rat larynx.
The larynges of Sprague Dawley rats of embryonic day (E) 13, 15, 17, 19, 21, postnatal day 0, 14, and adult of 250 gm were collected. Four larynges of each age were harvested, cut into 15-μm serial sections, stained with hematoxylin and eosin, and evaluated under light microscopy. Representative digital images were recorded and evaluated at the preglottic (supraglottic in humans), glottic, and postglottic (subglottic in humans) levels.
Brachial arches were observed at E13. At E17, immature structures of the larynx, including skeletal muscle, cartilage, and the lumen were identifiable. Chondrification and muscle formation were clearly seen by E19. The muscular and cartilagenous components of the larynx were well established by E21. During the span between birth and adult maturation, the size of the larynx increased from a height of 1.10 mm to 2.90 mm, and from a width of 1.80 mm to 5.40 mm, and from a length of 1.38 mm to 4.77 mm in the stained section. Although developed at E21, the laryngeal structures continued to grow by approximately 30%.
Rat laryngeal development parallels that in mice and humans. In the rat, at E17 immature structures of the larynx are identifiable, they are well developed at birth and grow by approximately 30% into adulthood. Understanding the chronology and morphology of the embryogenesis of the rat laryngeal musculature is essential and will allow for further evaluation of the embryologic innervation of these muscles.
Level of Evidence
N/A. Laryngoscope, 123:3093–3098, 2013