The authors have no other funding, financial relationships, or conflicts of interest to disclose.
Pathological study of otitis media with effusion after treatment with intranasal pulmonary surfactant
Article first published online: 5 AUG 2013
Copyright © 2013 The American Laryngological, Rhinological and Otological Society, Inc.
Volume 123, Issue 12, pages 3148–3155, December 2013
How to Cite
Zhu, Z. H., Shan, Y. J., Han, Y., Zhu, L. W. and Ma, Z. X. (2013), Pathological study of otitis media with effusion after treatment with intranasal pulmonary surfactant. The Laryngoscope, 123: 3148–3155. doi: 10.1002/lary.24166
- Issue published online: 25 NOV 2013
- Article first published online: 5 AUG 2013
- Manuscript Accepted: 1 APR 2013
- Manuscript Revised: 24 FEB 2013
- Manuscript Received: 21 OCT 2012
- Scientific Program of Pudong Health Bureau of Shanghai, China. Grant Number: PW2009A-2
- Otitis media with effusion;
- pulmonary surfactant;
- eustachian tube;
- nasal drops;
To evaluate the histopathological effect of intranasal pulmonary surfactant (PS) on the eustachian tube (ET) in guinea pigs with otitis media with effusion (OME).
Randomized control trial.
Nonviable heat-killed Hemophilus influenzae solution was injected into the tympanum of guinea pigs by a trans-eardrum approach to establish OME. Guinea pigs were divided into four groups: normal controls (group A), untreated OME (group B), saline-treated (group C), PS-treated (group D). The response threshold of the guinea pigs was measured by auditory brainstem response (ABR), and data were analyzed by one-way analysis of variance. The histopathological changes in the osseous, cartilaginous, and muscular portions of the ET were observed systematically by light microscopy.
The ABR threshold in OME group B was raised significantly compared with normal group (A). The response in saline-treated group C was not statistically significantly different compared with OME group B. Seven days after intranasal dripping of pulmonary surfactant in PS-treated group D, the response threshold showed at statistically significant decrease compared with OME B and saline-treated C groups. In OME group B and saline-treated group C, mucosa showed swelling with goblet cell hyperplasia, and cilia were irregularly arranged. In PS-treated group D, there was slight mucosal swelling with fewer goblet cells, and cilia were regularly arranged, similar to the normal group A.
The findings of the study indicate that intranasal pulmonary surfactant drops have protective and hyposecretory effects on the mucociliary system of the ET in guinea pigs suffering from OME.
Level of Evidence
NA. Laryngoscope, 123:3148–3155, 2013