This work was funded in part by the EPSRC Centre for Innovative Manufacturing in Regenerative Medicine and the European Community's FP7 project Biodesign EUFP7-NMP.20102.3-1 (grant: 262948). The research leading to these results received funding from the European Research Council under the European Community's Seventh FP7 project FP72007-2013 (grant: 227845).
Development of a porous poly(DL-lactic acid-co-glycolic acid)-based scaffold for mastoid air-cell regeneration
Article first published online: 13 MAY 2013
© 2013 The Authors. The Laryngoscope is published by Wiley Periodicals, Inc. on behalf of The American Laryngological, Rhinological and Otological Society, Inc.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Volume 123, Issue 12, pages 3156–3161, December 2013
How to Cite
Gould, T. W. A., Birchall, J. P., Mallick, A. S., Alliston, T., Lustig, L. R., Shakesheff, K. M. and Rahman, C. V. (2013), Development of a porous poly(DL-lactic acid-co-glycolic acid)-based scaffold for mastoid air-cell regeneration. The Laryngoscope, 123: 3156–3161. doi: 10.1002/lary.24173
The authors have no other funding, financial relationships, or conflicts of interest to disclose.
- Issue published online: 25 NOV 2013
- Article first published online: 13 MAY 2013
- Manuscript Accepted: 2 APR 2013
- Manuscript Revised: 8 MAR 2013
- Manuscript Received: 19 NOV 2012
- poly(DL-lactic acid-co-glycolic acid);
To develop a porous, biodegradable scaffold for mastoid air-cell regeneration.
In vitro development of a temperature-sensitive poly(DL-lactic acid-co-glycolic acid)/poly(ethylene glycol) (PLGA/PEG) scaffold tailored for this application.
Human mastoid bone microstructure and porosity were investigated using micro-computed tomography. PLGA/PEG-alginate scaffolds were developed, and scaffold porosity was assessed. Human bone marrow mesenchymal stem cells (hBM-MSCs) were cultured on the scaffolds in vitro. Scaffolds were loaded with ciprofloxacin, and release of ciprofloxacin over time in vitro was assessed.
Porosity of human mastoid bone was measured at 83% with an average pore size of 1.3 mm. PLGA/PEG-alginate scaffold porosity ranged from 43% to 78% depending on the alginate bead content. The hBM-MSCs proliferate on the scaffolds in vitro, and release of ciprofloxacin from the scaffolds was demonstrated over 7 to 10 weeks.
The PLGA/PEG-alginate scaffolds developed in this study demonstrate similar structural features to human mastoid bone, support cell growth, and display sustained antibiotic release. These scaffolds may be of potential clinical use in mastoid air-cell regeneration. Further in vivo studies to assess the suitability of PLGA/PEG-alginate scaffolds for this application are required
Level of Evidence
N/A. Laryngoscope, 123:3156–3161, 2013