Mouse cochleostomy: A minimally invasive dorsal approach for modeling cochlear implantation

Authors

  • Hakan Soken MD,

    1. Department of Otolaryngology Head and Neck Surgery, University of Iowa, Iowa City, Iowa, U.S.A
    Current affiliation:
    1. Department of Otolaryngology, Eskisehir Military Hospital, Eskisehir, Turkey
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  • Barbara K. Robinson MS,

    1. Department of Otolaryngology Head and Neck Surgery, University of Iowa, Iowa City, Iowa, U.S.A
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  • Shawn S. Goodman PhD,

    1. Department of Communication Sciences and Disorders, University of Iowa, Iowa City, Iowa, U.S.A.
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  • Paul J. Abbas PhD,

    1. Department of Otolaryngology Head and Neck Surgery, University of Iowa, Iowa City, Iowa, U.S.A
    2. Department of Communication Sciences and Disorders, University of Iowa, Iowa City, Iowa, U.S.A.
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  • Marlan R. Hansen MD,

    Corresponding author
    1. Department of Otolaryngology Head and Neck Surgery, University of Iowa, Iowa City, Iowa, U.S.A
    • Send correspondence to Marlan R. Hansen, MD, 200 Hawkins Dr, 21163 PFP, Iowa City, IA, 52242-1078. E-mail: marlan-hansen@uiowa.edu

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  • Jonathan C. Kopelovich MD

    1. Department of Otolaryngology Head and Neck Surgery, University of Iowa, Iowa City, Iowa, U.S.A
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  • The authors have no other funding, financial relationships, or conflicts of interest to disclose.

  • This study was supported by NIH T32 DC000040 grant as well as NIDCD-P30 DC 010362.

Abstract

Objectives/Hypothesis

The murine model has been used extensively to model and study human deafness. Technical difficulty in the surgical approach due to the small size of the tympanic bulla and a robust stapedial artery has limited its application for studies of cochlear implantation and electrical stimulation. We describe a minimally traumatic, stapedial artery–sparing approach to the round window that may be used to access the mouse cochlea for acute or chronic studies of implantation and stimulation.

Study Design

Animal model.

Methods

Fifteen C57BL6J mice were used to validate this approach. Auditory brainstem response threshold and distortion product otoacoustic emissions were obtained preoperatively and 2 weeks postoperatively to determine hearing preservation results.

Results

The approach provided excellent exposure for round-window implantation. Substantial hearing was preserved in all animals with a mean postimplantation auditory brainstem response threshold increase of 27.8 dB. Otoacoustic emissions were lost in subjects with the largest threshold shifts.

Conclusions

Residual hearing after cochlear implantation is a determinant of success both with standard cochlear implant electrodes and with electrodes designed to optimize hearing preservation. Here, we have preserved usable hearing after implantation of C57BL6J mice, an endogenous model of human presbycusia. The murine model may become a powerful tool to assay the effects of cochlear intervention in different genetic backgrounds. Laryngoscope, 123:E109–E115, 2013

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