This study was supported by Grants-in-Aid for Scientific Research (B) and Grants-in-Aid for Young Scientists (B) from The Ministry of Education, Culture, Sports, Science and Technology, Japan; the Adaptable and Seamless Technology Transfer Program Through Target-Driven R-D from the Japan Science and Technology Agency; and Fukushima Medical University.
Effective embryoid body formation from induced pluripotent stem cells for regeneration of respiratory epithelium
Version of Record online: 1 NOV 2013
© 2013 The American Laryngological, Rhinological and Otological Society, Inc.
Volume 124, Issue 1, pages E8–E14, January 2014
How to Cite
Otsuki, K., Imaizumi, M., Nomoto, Y., Nomoto, M., Wada, I., Miyake, M. and Omori, K. (2014), Effective embryoid body formation from induced pluripotent stem cells for regeneration of respiratory epithelium. The Laryngoscope, 124: E8–E14. doi: 10.1002/lary.24201
Editor's Note: This Manuscript was accepted for publication April 4, 2013.
Presented at the Annual Meeting of the American Laryngological Association, Orlando, Florida, U.S.A., April 10–11, 2013.
The authors have no other funding, financial relationships, or conflicts of interest to disclose.
- Issue online: 20 DEC 2013
- Version of Record online: 1 NOV 2013
- Accepted manuscript online: 20 MAY 2013 03:15AM EST
- Manuscript Revised: 4 APR 2013
- Manuscript Accepted: 4 APR 2013
- Manuscript Received: 28 FEB 2013
- Scientific Research (B) from The Ministry of Education, Culture, Sports, Science and Technology, Japan
- Adaptable and Seamless Technology Transfer Program Through Target-Driven R&D from the Japan Science and Technology Agency
- Fukushima Medical University
- Induced pluripotent stem cells;
- embryoid bodies;
- ciliated cells;
- respiratory epithelium
We have previously demonstrated the potential use of induced pluripotent stem (iPS) cells for regeneration of respiratory epithelium by culturing embryoid bodies (EBs). The aim of the present study was to determine the most effective conditions for EB formation from iPS cells for regeneration of respiratory epithelium.
iPS cells cultured on a gelatin-coated dish were seeded on low-attachment plates for generating EBs. Under several conditions including the air–liquid interface (ALI) method, with varying cell numbers and suspension times, EBs were transferred to a gelatin-coated dish supplemented with growth factors. The shape, size, aggregation, and adhesion of EBs for iPS cell differentiation were evaluated, and the cultured tissue was histologically examined.
EBs appropriate for differentiation were observed using 1,000 cells after 5 days of suspension culture. Respiratory epithelium-like tissue was histologically observed. The ciliary epithelium was confirmed immunohistologically.
Based on the varying suspension times and cell numbers with the ALI method, this study presented effective conditions for EB formation from iPS cells for regeneration of respiratory epithelium.
Level of Evidence
NA. Laryngoscope, 124:E8–E14, 2014