Source of funding: The University of Adelaide and the European Rhinologic Society. P.J.W. receives royalties from Medtronic for instrument designed and is a consultant for Neilmed Pharmaceuticals Pty Ltd. The authors have no other funding, financial relationships, or conflicts of interest to disclose.
Safety evaluation of a sinus surfactant in an explant-based cytotoxicity assay
Article first published online: 9 JUL 2013
© 2013 The American Laryngological, Rhinological and Otological Society, Inc.
Volume 124, Issue 2, pages 369–372, February 2014
How to Cite
Tan, N. C.-W., Cooksley, C. M., Paramasivan, S., Vreugde, S. and Wormald, P.-J. (2014), Safety evaluation of a sinus surfactant in an explant-based cytotoxicity assay. The Laryngoscope, 124: 369–372. doi: 10.1002/lary.24255
- Issue published online: 21 JAN 2014
- Article first published online: 9 JUL 2013
- Accepted manuscript online: 18 JUN 2013 03:40AM EST
- Manuscript Accepted: 22 APR 2013
- Manuscript Revised: 7 MAR 2013
- Manuscript Received: 13 NOV 2012
- Chronic rhinosinusitis;
- sinus surfactant;
- human sinonasal explants;
Biofilms are associated with clinical relapse following surgery for chronic rhinosinusitis. Encased bacteria are protected from innate immunity and antimicrobial therapy. Surfactants can disperse the biofilm into its planktonic phenotype so that traditional treatments may be effective. The aim of this study was to assess a surfactant for its cytotoxicity profile.
In vitro explant-based cytotoxicity study.
Sinonasal mucosa harvested from patients undergoing sinus surgery was tested using an air-liquid interface explant system. Surfactant at 1×, 2×, and 3× manufacturer's recommended concentrations were compared to control (saline) and Zinc Sulphate (ZnSO4), a known cytotoxic agent. Culture supernatant was analyzed for lactate dehydrogenase (LDH) as a marker of cellular toxicity. After 7 days, specimens were imaged using structured histopathology and scanning electron microscopy.
Application of surfactant at 1× concentration did not elicit an elevation in LDH, whereas ZnSO4 caused a significant rise 1 day after application. Specimens tested with a 2× and 3× surfactant demonstrated LDH rises 4 days and 2 days after application, respectively. Mucosa tested with the 1× surfactant and control demonstrated intact cellular structures on histopathology and preserved cilial ultrastructure on SEM. In ZnSO4-treated specimens, marked cellular degradation and ciliary denudation occurred.
The surfactant does not appear to elicit cellular toxicity using an in vitro explant model at the manufacturer's recommended concentration. At higher concentrations, there may be dose-related toxicity that requires further investigation. In vivo testing is required to prove its efficacy in the treatment of recalcitrant chronic rhinosinusitis.
Level of Evidence
N/A. Laryngoscope, 124:369–372, 2014